<p>In this study, hydrogel microparticles of sodium alginate and natural zeolite – clinoptilolite enriched with silver ions were synthesized, and a zeolitic tuff from the Zlatokop mine (Vranjska Banja) containing clinoptilolite (73&#xa0;wt%), quartz (10 wt%) and feldspar (7 wt%) was used. Zeolite phase from the tuff was enriched with silver ions using ion exchange method. For the synthesis, optimal parameters were determined as follows: suspension of zeolite enriched with silver ions (Ag-Z; 2.0 wt%) in water solution of sodium alginate (1.5 wt%) and 0.25 mol dm<sup>−3</sup> solution of zinc chloride as a crosslinker. The prepared composite microparticles were characterized using Fourier-transform infrared spectroscopy (FT-IR analysis), thermal analysis (TG/DTG), swelling studies at pH = 7.4 and room temperature, and optical microscopy. Additionally, the desorption behavior of silver ions from the composites was investigated. Antibacterial activity was tested against Gram-negative bacterium <i>Escherichia coli</i> DSM 498 and Gram-positive bacterium <i>Staphylococcus aureus</i> ATCC 25923 by a disc diffusion method. The hydrogel microparticles with 2.0 wt% Ag-Z showed superior antibacterial properties compared to other synthesized samples.</p>

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Antibacterial Activity of Hydrogel Microparticles Based on Alginate/Silver Enriched Natural Zeolite Composites

  • Milica Janković,
  • Jelena Dikić,
  • Nemanja Vučković,
  • Nedeljko Milosavljević,
  • Melina Kalagasidis Krušić,
  • Nikola Milašinović

摘要

In this study, hydrogel microparticles of sodium alginate and natural zeolite – clinoptilolite enriched with silver ions were synthesized, and a zeolitic tuff from the Zlatokop mine (Vranjska Banja) containing clinoptilolite (73 wt%), quartz (10 wt%) and feldspar (7 wt%) was used. Zeolite phase from the tuff was enriched with silver ions using ion exchange method. For the synthesis, optimal parameters were determined as follows: suspension of zeolite enriched with silver ions (Ag-Z; 2.0 wt%) in water solution of sodium alginate (1.5 wt%) and 0.25 mol dm−3 solution of zinc chloride as a crosslinker. The prepared composite microparticles were characterized using Fourier-transform infrared spectroscopy (FT-IR analysis), thermal analysis (TG/DTG), swelling studies at pH = 7.4 and room temperature, and optical microscopy. Additionally, the desorption behavior of silver ions from the composites was investigated. Antibacterial activity was tested against Gram-negative bacterium Escherichia coli DSM 498 and Gram-positive bacterium Staphylococcus aureus ATCC 25923 by a disc diffusion method. The hydrogel microparticles with 2.0 wt% Ag-Z showed superior antibacterial properties compared to other synthesized samples.