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<p>Tyrosinases (E.C. 1.14.18.1) are enzymes widely distributed in fungi that catalyze the ortho-hydroxylation of monophenols to diphenols and the oxidation of diphenols to quinones and present a wide spectrum of phenolic compounds as substrates and are among enzymes with high potential for industrial applications. We present here the screening of tyrosinases-producing fungi isolated in the Paranaense rainforest of Argentina, as well as the intracellular and extracellular expression of this enzyme in three culture media. Additionally, we analyzed the mycelial proteome of <i>P. brevispora</i> BAFC 633 and its secretome in liquid media, aiming to explore differences in the expression profile of tyrosinases and other endogenous proteins that may interfere with this enzymatic activity<i>. P. brevispora</i> BAFC633 presented higher tyrosinase secretion, showing enhanced enzymatic activity when mycelium is cultured on potato medium. A peak in activity was observed, in each experiment, between days 6 and 8 of incubation period, followed by a precipitous decline in activity that was experimentally attributed to the secretion of proteases into the medium. Finally, biotechnological implications of the results are discussed.</p>

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Exploring Novel Tyrosinase Enzymes in Basidiomycota Fungi from Paranaense Rainforest (Argentina)

  • 期刊论文

  • 发表日期：

    2025年6月11日

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  • Noelia Malena Schröder,
  • Aníbal Sebastián Chelaliche,
  • María Daniela Rodríguez,
  • María Isabel Fonseca,
  • Pedro Darío Zapata

摘要

Tyrosinases (E.C. 1.14.18.1) are enzymes widely distributed in fungi that catalyze the ortho-hydroxylation of monophenols to diphenols and the oxidation of diphenols to quinones and present a wide spectrum of phenolic compounds as substrates and are among enzymes with high potential for industrial applications. We present here the screening of tyrosinases-producing fungi isolated in the Paranaense rainforest of Argentina, as well as the intracellular and extracellular expression of this enzyme in three culture media. Additionally, we analyzed the mycelial proteome of P. brevispora BAFC 633 and its secretome in liquid media, aiming to explore differences in the expression profile of tyrosinases and other endogenous proteins that may interfere with this enzymatic activity. P. brevispora BAFC633 presented higher tyrosinase secretion, showing enhanced enzymatic activity when mycelium is cultured on potato medium. A peak in activity was observed, in each experiment, between days 6 and 8 of incubation period, followed by a precipitous decline in activity that was experimentally attributed to the secretion of proteases into the medium. Finally, biotechnological implications of the results are discussed.