Scholarlink

<p>Despite extensive characterization of its biochemical properties, <i>Acinetobacter haemolyticus</i> recombinant alkaline-stable lipase KV1 (Lip-KV1) remains unexplored in crosslinked enzyme aggregates (CLEAs) as a means to enhance its catalytic performance. This research focused on optimizing the preparation of Lip-KV1CLEAs through the application of response surface methodology and Box–Behnken design (BBD) to optimize the parameters, glutaraldehyde concentration (mM), Tween 80 (v/v%), pH, and temperature, for the response of recovery activity (%). Results revealed that the CLEAs-Lip-KV1 exhibited the highest recovery activity of 123.3% under the following optimum condition: isopropanol as the precipitant (80% v/v), Tween 80 at 0.01% (v/v), 60 mM glutaraldehyde, agitation at 200 rpm, pH 4.0 with a 2-h crosslinking duration at 30 °C. The resultant CLEAs-Lip-KV1 was stable in 10% v/v of methanol. Notably, the optimum temperature and pH stability of the CLEAs-Lip-KV1 was elevated compared to its free form, from the corresponding 40 °C and pH 8, to 50 °C and pH 10, with its thermal stability reaching up to 80 °C. The FTIR spectrum and FESEM micrographs confirmed these changes chemically and morphologically, which verified the CLEAs-Lip-KV1 preparation.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Statistically Assisted Preparation of Novel Alkaline Recombinant Crosslinked Enzyme Aggregates Lipase KV1 and Their Physiochemical Attributes

  • 期刊论文

  • 发表日期：

    2025年1月9日

查看全文
  • Oumaima Maroua Bouguerra,
  • Roswanira Abdul Wahab,
  • Fahrul Huyop,
  • Naji Arafat Mahat,
  • Evi Susanti

摘要

Despite extensive characterization of its biochemical properties, Acinetobacter haemolyticus recombinant alkaline-stable lipase KV1 (Lip-KV1) remains unexplored in crosslinked enzyme aggregates (CLEAs) as a means to enhance its catalytic performance. This research focused on optimizing the preparation of Lip-KV1CLEAs through the application of response surface methodology and Box–Behnken design (BBD) to optimize the parameters, glutaraldehyde concentration (mM), Tween 80 (v/v%), pH, and temperature, for the response of recovery activity (%). Results revealed that the CLEAs-Lip-KV1 exhibited the highest recovery activity of 123.3% under the following optimum condition: isopropanol as the precipitant (80% v/v), Tween 80 at 0.01% (v/v), 60 mM glutaraldehyde, agitation at 200 rpm, pH 4.0 with a 2-h crosslinking duration at 30 °C. The resultant CLEAs-Lip-KV1 was stable in 10% v/v of methanol. Notably, the optimum temperature and pH stability of the CLEAs-Lip-KV1 was elevated compared to its free form, from the corresponding 40 °C and pH 8, to 50 °C and pH 10, with its thermal stability reaching up to 80 °C. The FTIR spectrum and FESEM micrographs confirmed these changes chemically and morphologically, which verified the CLEAs-Lip-KV1 preparation.