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<p>The purpose of this study was to fabricate the nanocomposite hydrogels based on polyethylene glycol-poly caprolactone-polyethylene glycol containing montmorillonite nanoparticles (PEG-PCL-PEG/MMT) for bone tissue engineering application. The physical properties of the synthesized copolymer and the nanocomposite hydrogels were analyzed by Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (H-NMR), field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), swelling ratio analysis, and rheology analysis. Based on the results of FTIR and H-NMR analyses, the synthesis of the PEG-PCL-PEG copolymer was successfully performed. Likewise, the presence of montmorillonite nanoparticles in the hydrogel structure was confirmed by XRD analysis. FESEM and EDAX analyses showed that the montmorillonite nanoparticles were well dispersed in the hydrogel matrix without significant no agglomeration formation. According to the results of equilibrium swelling analysis, the PEG-PCL-PEG/MMT nanocomposite hydrogel had higher water absorption capability compared with that of bare copolymer, which increases by adding more montmorillonite nanoparticles content into copolymer matrix. The bioactivity of the samples after 7 days of immersion in simulated body fluid (SBF) was evaluated by FESEM analysis, and, the biocompatibility of the samples was assessed by MTT assay. The results of FESEM-EDX analysis confirmed the formation of hydroxyapatite structure on the surface of the samples after immersion in SBF for 7 days, indicating the bioactivity of the nanocomposite hydrogels. Additionally, MTT findings showed appropriate cell viability in comparison with that of control sample on days 3 and 7, indicating the suitable biocompatibility of the PEG-PCL-PEG nanocomposite hydrogels. Based on the current results, this designed nanocomposite hydrogel can be a suitable candidate for bone tissue engineering application.</p>

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Fabrication and Characterization of Injectable Nanocomposite Hydrogel Containing PEG-PCL-PEG Copolymer/Montmorillonite Nanoparticles for Bone Tissue Regeneration

  • 期刊论文

  • 发表日期：

    2025年1月16日

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  • Faezeh Afkhami,
  • Hossein Eslami,
  • Alireza Sabzevari,
  • Mojtaba Ansari

摘要

The purpose of this study was to fabricate the nanocomposite hydrogels based on polyethylene glycol-poly caprolactone-polyethylene glycol containing montmorillonite nanoparticles (PEG-PCL-PEG/MMT) for bone tissue engineering application. The physical properties of the synthesized copolymer and the nanocomposite hydrogels were analyzed by Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (H-NMR), field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), swelling ratio analysis, and rheology analysis. Based on the results of FTIR and H-NMR analyses, the synthesis of the PEG-PCL-PEG copolymer was successfully performed. Likewise, the presence of montmorillonite nanoparticles in the hydrogel structure was confirmed by XRD analysis. FESEM and EDAX analyses showed that the montmorillonite nanoparticles were well dispersed in the hydrogel matrix without significant no agglomeration formation. According to the results of equilibrium swelling analysis, the PEG-PCL-PEG/MMT nanocomposite hydrogel had higher water absorption capability compared with that of bare copolymer, which increases by adding more montmorillonite nanoparticles content into copolymer matrix. The bioactivity of the samples after 7 days of immersion in simulated body fluid (SBF) was evaluated by FESEM analysis, and, the biocompatibility of the samples was assessed by MTT assay. The results of FESEM-EDX analysis confirmed the formation of hydroxyapatite structure on the surface of the samples after immersion in SBF for 7 days, indicating the bioactivity of the nanocomposite hydrogels. Additionally, MTT findings showed appropriate cell viability in comparison with that of control sample on days 3 and 7, indicating the suitable biocompatibility of the PEG-PCL-PEG nanocomposite hydrogels. Based on the current results, this designed nanocomposite hydrogel can be a suitable candidate for bone tissue engineering application.