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<p>We present a fast and reliable genetic method for sexing Pampas deer (<i>Ozotoceros bezoarticus</i>) via high-resolution melting (HRM) analysis of amplicons obtained through real-time polymerase chain reaction (PCR-RT). This method employs the KY1/KY2 primer set to amplify an informative fragment of the <i>Amelogenin</i> gene that accurately determines the sex of Pampas deer. This approach is effective for analyzing noninvasive samples with low DNA quality and quantity. Additionally, this new PCR-RT protocol can be easily adapted for sexing non-invasive samples from other deer and ungulate species.</p>

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Sex determination of the Pampas deer (Ozotoceros bezoarticus) via high-resolution melting analysis

  • 期刊论文

  • 发表日期：

    2025年7月3日

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  • Guillermo Morera,
  • Mariana Cosse,
  • Susana González

摘要

We present a fast and reliable genetic method for sexing Pampas deer (Ozotoceros bezoarticus) via high-resolution melting (HRM) analysis of amplicons obtained through real-time polymerase chain reaction (PCR-RT). This method employs the KY1/KY2 primer set to amplify an informative fragment of the Amelogenin gene that accurately determines the sex of Pampas deer. This approach is effective for analyzing noninvasive samples with low DNA quality and quantity. Additionally, this new PCR-RT protocol can be easily adapted for sexing non-invasive samples from other deer and ungulate species.