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<p><i>Dastarcus kurosawai</i> Sasaji (Bothrideridae: Coleoptera), endemic to the Nansei Islands of Japan, is considered a promising natural enemy for the augmentative biological control of <i>Monochamus alternatus</i> Hope (Coleoptera: Cerambycidae). However, mass-rearing methods for this parasitoid have yet to be fully established. In this study, we evaluated once-frozen <i>Zophobas atratus</i> Fabricius (Coleoptera: Tenebrionidae) pupae as an alternative host and estimated the optimal inoculation density for mass rearing. Parasitism rates were significantly higher using once-frozen <i>Z. atratus</i> pupae than fresh <i>Z. atratus</i> pupae and fresh <i>Psacothea hilaris</i> Pascoe (Coleoptera: Cerambycidae) pupae. Adults reared on once-frozen pupae had slightly shorter forewings than those from <i>P. hilaris</i>, but other developmental traits were comparable. Pupation success and pupal size declined at a 40-larval density, while no significant adverse effects on body size were observed at 10–20 larvae per host. However, at a 20-larval density, the parasitism success rate tended to decrease. Based on these results, we conclude that once-frozen <i>Z. atratus</i> pupae are suitable as a substitute host for mass rearing of <i>D. kurosawai</i>, and that approximately 10 larvae per host represents the optimal inoculation density for balancing production efficiency, parasitism success, and parasitoid quality.</p>

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Rearing of parasitoid beetle Dastarcus kurosawai (Bothrideridae: Coleoptera) larvae using once-frozen Zophobas atratus (Coleoptera: Tenebrionidae) pupae

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  • 发表日期：

    2026年1月12日

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  • Tianyi Zheng,
  • Yuta Yamaguchi,
  • Kyo Itoyama

摘要

Dastarcus kurosawai Sasaji (Bothrideridae: Coleoptera), endemic to the Nansei Islands of Japan, is considered a promising natural enemy for the augmentative biological control of Monochamus alternatus Hope (Coleoptera: Cerambycidae). However, mass-rearing methods for this parasitoid have yet to be fully established. In this study, we evaluated once-frozen Zophobas atratus Fabricius (Coleoptera: Tenebrionidae) pupae as an alternative host and estimated the optimal inoculation density for mass rearing. Parasitism rates were significantly higher using once-frozen Z. atratus pupae than fresh Z. atratus pupae and fresh Psacothea hilaris Pascoe (Coleoptera: Cerambycidae) pupae. Adults reared on once-frozen pupae had slightly shorter forewings than those from P. hilaris, but other developmental traits were comparable. Pupation success and pupal size declined at a 40-larval density, while no significant adverse effects on body size were observed at 10–20 larvae per host. However, at a 20-larval density, the parasitism success rate tended to decrease. Based on these results, we conclude that once-frozen Z. atratus pupae are suitable as a substitute host for mass rearing of D. kurosawai, and that approximately 10 larvae per host represents the optimal inoculation density for balancing production efficiency, parasitism success, and parasitoid quality.