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<p>Portions of the sodium channel gene were sequenced for a strain of <i>Neoseiulus womersleyi</i> (Schicha) (Acari:Phytoseiidae) showing resistance to a pyrethroid. The results revealed that the strain had M918L and S1539T. The compositions of phytoseiid mite species inhabiting apple orchards in Iwate prefecture, northern Japan, were also examined in 2023 and 2024 using quantitative sequencing, species-specific PCR, and nucleotide sequencing. Results revealed the most dominant species in the apple orchards as <i>Typhlodromus vulgaris</i> Ehara (Acari:Phytoseiidae), followed by <i>N. womersleyi</i>. Also observed were <i>Neoseiulus makuwa</i> (Ehara) (Acari:Phytoseiidae), <i>Scapulaseius okinawanus</i> (Ehara) (Acari:Phytoseiidae), <i>Amblyseius tsugawai</i> Ehara (Acari:Phytoseiidae), and <i>Amblyseius andersoni</i> (Chant) (Acari:Phytoseiidae), but their relative proportions were low. Nucleotide sequencing of the individually amplified sodium channel gene fragments showed M918L as distributed widely in <i>N. womersleyi</i> and showed L925V/M distributed in <i>T. vulgaris</i> in some apple orchards. These results indicate that indigenous phytoseiid mite species in apple orchards might have reduced sensitivity to pyrethroids.</p>

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Sodium channel mutations (M918L and L925V/M) in phytoseiid mite species and their distribution in apple orchards

  • 期刊论文

  • 发表日期：

    2025年7月4日

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  • Shoji Sonoda,
  • Yuri Yoshida,
  • Masaki Kato,
  • Hidenari Kishimoto,
  • Masatoshi Toyama

摘要

Portions of the sodium channel gene were sequenced for a strain of Neoseiulus womersleyi (Schicha) (Acari:Phytoseiidae) showing resistance to a pyrethroid. The results revealed that the strain had M918L and S1539T. The compositions of phytoseiid mite species inhabiting apple orchards in Iwate prefecture, northern Japan, were also examined in 2023 and 2024 using quantitative sequencing, species-specific PCR, and nucleotide sequencing. Results revealed the most dominant species in the apple orchards as Typhlodromus vulgaris Ehara (Acari:Phytoseiidae), followed by N. womersleyi. Also observed were Neoseiulus makuwa (Ehara) (Acari:Phytoseiidae), Scapulaseius okinawanus (Ehara) (Acari:Phytoseiidae), Amblyseius tsugawai Ehara (Acari:Phytoseiidae), and Amblyseius andersoni (Chant) (Acari:Phytoseiidae), but their relative proportions were low. Nucleotide sequencing of the individually amplified sodium channel gene fragments showed M918L as distributed widely in N. womersleyi and showed L925V/M distributed in T. vulgaris in some apple orchards. These results indicate that indigenous phytoseiid mite species in apple orchards might have reduced sensitivity to pyrethroids.