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<p>Roses (<i>Rosa</i> spp.) are economically important ornamental plants in Vietnam and are susceptible to a range of soil-borne pathogens, including plant-parasitic nematodes. During a survey of rose-growing areas in Vinh Phuc Province, root-lesion nematodes were detected on rose plants exhibiting reduced vigor, mild chlorosis, stunted growth, and necrotic lesions on feeder roots. <i>Pratylenchus coffeae</i> was detected in 25% of the collected samples, with mean population densities of 16 individuals per 250&#xa0;g of soil and 45 individuals per 5&#xa0;g of roots. Morphological and morphometric characteristics of the recovered population were consistent with <i>Pratylenchus coffeae,</i> including a slightly offset lip region with two annuli, a robust stylet with rounded basal knobs, a lateral field with four incisures, a large spermatheca usually filled with sperm, and a short subcylindrical tail with a smooth to crenate terminus. Molecular analyses based on the D2–D3 expansion segments of 28S rDNA, ITS rDNA, and COI mtDNA confirmed species identity, showing 99.0–100% similarity with reference sequences in GenBank database. Phylogenetic and haplotype network analyses consistently placed the Vietnamese population within a well-supported <i>P. coffeae</i> clade, with low genetic variation and no clear geographic structuring. This study represents the first report of <i>P. coffeae</i> associated with rose in Vietnam, expanding the known host range of this species in the country.</p>

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First report of Pratylenchus coffeae on rose (Rosa spp.) in Vietnam, with morphological and molecular confirmation

  • 期刊论文

  • 发表日期：

    2026年7月29日

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  • Thi Duyen Nguyen,
  • Thi Mai Linh Le,
  • Manh Ha Nguyen,
  • Luu Thu Thuy Hoang,
  • Quang Phap Trinh

摘要

Roses (Rosa spp.) are economically important ornamental plants in Vietnam and are susceptible to a range of soil-borne pathogens, including plant-parasitic nematodes. During a survey of rose-growing areas in Vinh Phuc Province, root-lesion nematodes were detected on rose plants exhibiting reduced vigor, mild chlorosis, stunted growth, and necrotic lesions on feeder roots. Pratylenchus coffeae was detected in 25% of the collected samples, with mean population densities of 16 individuals per 250 g of soil and 45 individuals per 5 g of roots. Morphological and morphometric characteristics of the recovered population were consistent with Pratylenchus coffeae, including a slightly offset lip region with two annuli, a robust stylet with rounded basal knobs, a lateral field with four incisures, a large spermatheca usually filled with sperm, and a short subcylindrical tail with a smooth to crenate terminus. Molecular analyses based on the D2–D3 expansion segments of 28S rDNA, ITS rDNA, and COI mtDNA confirmed species identity, showing 99.0–100% similarity with reference sequences in GenBank database. Phylogenetic and haplotype network analyses consistently placed the Vietnamese population within a well-supported P. coffeae clade, with low genetic variation and no clear geographic structuring. This study represents the first report of P. coffeae associated with rose in Vietnam, expanding the known host range of this species in the country.