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<p>Population genetics are routinely studied over vast geographical areas to address questions of origin, routes of migration and epidemiology. However, genetic diversity of certain microorganisms such as plant pathogenic fungi are difficult to study even on a small geographical scale due to their mixed reproduction systems, variable population sizes, clonality and versatility to host adaptation. The genetic variation of an economically important wheat fungal pathogen, <i>Pyrenophora tritici-repentis</i> (Ptr), was studied with isolates collected from a naturally infected wheat field located in Western Australia, using microsatellite (SSR) markers. Out of 142 Ptr isolates, 89 individuals showed unique multilocus haplotypes. On single leaves, individual disease lesions mostly consisted of genetically variable Ptr isolates, with very few clonal isolates. Distribution analysis of these indicated that wind-dispersal of Ptr conidia occurred over a small scale within individual leaves as well as longer dispersions of up to 45&#xa0;m. Race evaluation of the population via effector gene PCR assays defined 136 isolates as race 1 and six as race 2, and there was no indication of genetic groupings based on micro-locations or race. Population structure analysis suggests that the population consists of mixed migrants from long wind-dispersal or field management activities. Nucleotide sequence analysis of the homothallic mating-type genes, <i>MAT1-1</i> and <i>MAT1-2</i> revealed sequence conservation within the population. Altogether, the microscale evaluation of the single wheat field isolates demonstrated that the population is suitable for disease screening as it is representative of the wider Ptr population demographic in Australia.</p>

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Genetic variation and race structure in the tan spot wheat pathogen Pyrenophora tritici-repentis: a microscale assessment in a wheat field of Western Australia

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月29日

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  • Pao Theen See,
  • Catherine F. Cupitt,
  • Dion Bennett,
  • Caroline S. Moffat,
  • Simon R. Ellwood

摘要

Population genetics are routinely studied over vast geographical areas to address questions of origin, routes of migration and epidemiology. However, genetic diversity of certain microorganisms such as plant pathogenic fungi are difficult to study even on a small geographical scale due to their mixed reproduction systems, variable population sizes, clonality and versatility to host adaptation. The genetic variation of an economically important wheat fungal pathogen, Pyrenophora tritici-repentis (Ptr), was studied with isolates collected from a naturally infected wheat field located in Western Australia, using microsatellite (SSR) markers. Out of 142 Ptr isolates, 89 individuals showed unique multilocus haplotypes. On single leaves, individual disease lesions mostly consisted of genetically variable Ptr isolates, with very few clonal isolates. Distribution analysis of these indicated that wind-dispersal of Ptr conidia occurred over a small scale within individual leaves as well as longer dispersions of up to 45 m. Race evaluation of the population via effector gene PCR assays defined 136 isolates as race 1 and six as race 2, and there was no indication of genetic groupings based on micro-locations or race. Population structure analysis suggests that the population consists of mixed migrants from long wind-dispersal or field management activities. Nucleotide sequence analysis of the homothallic mating-type genes, MAT1-1 and MAT1-2 revealed sequence conservation within the population. Altogether, the microscale evaluation of the single wheat field isolates demonstrated that the population is suitable for disease screening as it is representative of the wider Ptr population demographic in Australia.