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<p><i>Nelumbo nucifera</i>, commonly known as the sacred lotus, has significant cultural, medicinal, and nutritional value as an aquatic plant in Vietnam. From 2022 to 2023, lotus plants in Hue City, Vietnam, exhibited symptoms of brown leaf blight. Initially, irregular light brown spots appeared on the leaves, which gradually expanded until the entire leaf turned dark brown and wilted. Based on morphological characteristics and DNA sequence data of three loci, the internal transcribed spacer (ITS), beta-tubulin (<i>TUB2</i>), and translation elongation factor 1-alpha (<i>TEF1-α</i>), supported by phylogenetic analysis, the pathogen was identified as <i>Nigrospora lacticolonia</i>. BLASTn analyses revealed 99.5–100% sequence similarity with <i>N. lacticolonia</i> accessions in GenBank. Multigene phylogenetic analysis based on a concatenated dataset of the three loci (ITS, <i>TUB2</i>, and <i>TEF1-α</i>) further clustered the isolates with <i>N. lacticolonia</i>, providing robust molecular support for this identification. Pathogenicity tests on whole plants grown under controlled conditions verified that the fungus was the causal agent of the disease, fulfilling Koch’s postulates. The disease incidence was approximately 91.67% (11/12) after 15 days post-inoculation, with an average lesion diameter of 121.27 ± 34.19&#xa0;mm and an average lesion length to leaf length ratio of 75.40%. To our knowledge, this is the first report of brown leaf blight caused by <i>N. lacticolonia</i> on <i>N. nucifera</i> in Vietnam and worldwide. These findings provide new insights into fungal pathogens affecting lotus and highlight the importance of developing effective disease management strategies to mitigate the economic impact of this disease on lotus cultivation.</p>

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First report of Nigrospora lacticolonia as the agent causing brown leaf blight disease on Nelumbo nucifera in Hue, Vietnam

  • 期刊论文

  • 发表日期：

    2026年4月14日

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  • Nguyen Quang Hoang Vu,
  • Hoang Thị Kim Hong,
  • Pham Thi Diem Thi,
  • Tran Thuy Lan,
  • Tran Thi Ngoc Tram,
  • Hoang Tan Quang

摘要

Nelumbo nucifera, commonly known as the sacred lotus, has significant cultural, medicinal, and nutritional value as an aquatic plant in Vietnam. From 2022 to 2023, lotus plants in Hue City, Vietnam, exhibited symptoms of brown leaf blight. Initially, irregular light brown spots appeared on the leaves, which gradually expanded until the entire leaf turned dark brown and wilted. Based on morphological characteristics and DNA sequence data of three loci, the internal transcribed spacer (ITS), beta-tubulin (TUB2), and translation elongation factor 1-alpha (TEF1-α), supported by phylogenetic analysis, the pathogen was identified as Nigrospora lacticolonia. BLASTn analyses revealed 99.5–100% sequence similarity with N. lacticolonia accessions in GenBank. Multigene phylogenetic analysis based on a concatenated dataset of the three loci (ITS, TUB2, and TEF1-α) further clustered the isolates with N. lacticolonia, providing robust molecular support for this identification. Pathogenicity tests on whole plants grown under controlled conditions verified that the fungus was the causal agent of the disease, fulfilling Koch’s postulates. The disease incidence was approximately 91.67% (11/12) after 15 days post-inoculation, with an average lesion diameter of 121.27 ± 34.19 mm and an average lesion length to leaf length ratio of 75.40%. To our knowledge, this is the first report of brown leaf blight caused by N. lacticolonia on N. nucifera in Vietnam and worldwide. These findings provide new insights into fungal pathogens affecting lotus and highlight the importance of developing effective disease management strategies to mitigate the economic impact of this disease on lotus cultivation.