Scholarlink

<p><i>Macrophomina</i> spp. are soil-borne phytopathogenic fungi within the division Ascomycota, known to cause significant yield losses in a wide range of crops, including sugar beet. In recent decades, root rot outbreaks associated with <i>Macrophomina</i> have increased in the Republic of Serbia, likely influenced by climate change and environmental stress. Despite this, there is limited information on the morphological and molecular characteristics of <i>Macrophomina</i> isolates from sugar beet. This study aimed to investigate the morphological and physiological variability of <i>Macrophomina</i> isolates, characterize them at the molecular level, and evaluate their genetic diversity using Simple Sequence Repeats (SSR) markers. The isolates of <i>Macrophomina</i> exhibit considerable phenotypic variation in colony morphology and chlorine sensitivity, suggesting inherent genetic diversity and potential differences in adaptation or survival under varying environmental conditions. Optimal growth occurred at 30–35&#xa0;°C, with pH tolerance between 4.0 and 7.0. Genetic diversity assessed using SSR markers revealed a relatively low level of variation among isolates. Principal Coordinates Analysis (PCoA) indicated that a few isolates formed distinct genetic clusters, indicating a limited population structure. These findings are essential for the development of resistant sugar beet cultivars and informed disease management practices in regions increasingly affected by <i>Macrophomina</i> outbreaks.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Morphological traits and SSR-Based genetic diversity of Macrophomina isolates causing charcoal rot in sugar beet in Serbia

  • 期刊论文

  • 发表日期：

    2026年3月19日

查看全文
  • Aleksandra Stankov Petreš,
  • Dragana Budakov,
  • Vera Stojšin,
  • Nevena Nagl,
  • Tatjana Dudaš,
  • Jelena Vukotić,
  • Mladen Petreš

摘要

Macrophomina spp. are soil-borne phytopathogenic fungi within the division Ascomycota, known to cause significant yield losses in a wide range of crops, including sugar beet. In recent decades, root rot outbreaks associated with Macrophomina have increased in the Republic of Serbia, likely influenced by climate change and environmental stress. Despite this, there is limited information on the morphological and molecular characteristics of Macrophomina isolates from sugar beet. This study aimed to investigate the morphological and physiological variability of Macrophomina isolates, characterize them at the molecular level, and evaluate their genetic diversity using Simple Sequence Repeats (SSR) markers. The isolates of Macrophomina exhibit considerable phenotypic variation in colony morphology and chlorine sensitivity, suggesting inherent genetic diversity and potential differences in adaptation or survival under varying environmental conditions. Optimal growth occurred at 30–35 °C, with pH tolerance between 4.0 and 7.0. Genetic diversity assessed using SSR markers revealed a relatively low level of variation among isolates. Principal Coordinates Analysis (PCoA) indicated that a few isolates formed distinct genetic clusters, indicating a limited population structure. These findings are essential for the development of resistant sugar beet cultivars and informed disease management practices in regions increasingly affected by Macrophomina outbreaks.