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<p>Being the third-largest producer of the globe, Bangladesh's food and economic security is intrinsically linked to rice production. Diseases are a major threat to rice production worldwide, including Bangladesh. Rice blast and bacterial blight (BB) pose significant global threats to rice cultivation. We employed marker-assisted backcrossing to successfully introgress four resistance (R) genes, i.e., <i>Pi9</i>, <i>Pb1</i>, <i>Xa4</i>, and <i>Xa21</i>, into the genetic background of a susceptible rice cultivar BRRI dhan28 to increase its resistance. Pi9-US2 and Pb1-US2 were used as donors for <i>Pi9</i> and <i>Pb1</i> blast-resistant genes, respectively, while IRBB58 was the source for <i>Xa4</i> and <i>Xa21</i> BB-resistant genes. A rigorous breeding program involving successive backcrosses, self-pollination, and marker-assisted selection was implemented to generate F<sub>1</sub> to BC<sub>3</sub>F<sub>7</sub> populations. Genotypic and phenotypic analyses of 400 BC<sub>3</sub>F<sub>2</sub> populations demonstrated simple Mendelian inheritance patterns of blast and BB resistance. Among the 50 best selected fixed advanced BC<sub>3</sub>F<sub>7</sub> lines, 37 exhibited resistances conferred by all four genes. The aAdvanced lines (ALs) exhibited a significantly low disease score (0–1) for rice blast and BB compared to the BRRI dhan28 (7–9). Marker-trait association analysis showed molecular markers negatively correlated with disease susceptibility. The multi-location trial demonstrated the broad-spectrum resistance of the selected ALs against blast and BB with improved yield and quality traits. Among the ALs, BR(Path)13784-BC3-63–6-4-HR6 or G35 gave the maximum average yield (6.74 t ha<sup>−1</sup>) across the locations. The molecular markers used in this study can be a promising tool for candidate gene identification. Our advanced lines have the potential to be directly released as a commercial variety or used as a genetic stock in rice breeding programs to combine with other genes.</p>

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Introgression of multiple resistance genes against blast and bacterial blight diseases in the background of mega variety BRRI dhan28

  • 期刊论文

  • 发表日期：

    2025年11月14日

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  • Mohammad Abdul Latif,
  • Lutfur Rahman,
  • Sheikh Arafat Islam Nihad,
  • Md. Omar Kayess,
  • Nazmul Islam,
  • Mohammad Ashik Iqbal Khan

摘要

Being the third-largest producer of the globe, Bangladesh's food and economic security is intrinsically linked to rice production. Diseases are a major threat to rice production worldwide, including Bangladesh. Rice blast and bacterial blight (BB) pose significant global threats to rice cultivation. We employed marker-assisted backcrossing to successfully introgress four resistance (R) genes, i.e., Pi9, Pb1, Xa4, and Xa21, into the genetic background of a susceptible rice cultivar BRRI dhan28 to increase its resistance. Pi9-US2 and Pb1-US2 were used as donors for Pi9 and Pb1 blast-resistant genes, respectively, while IRBB58 was the source for Xa4 and Xa21 BB-resistant genes. A rigorous breeding program involving successive backcrosses, self-pollination, and marker-assisted selection was implemented to generate F1 to BC3F7 populations. Genotypic and phenotypic analyses of 400 BC3F2 populations demonstrated simple Mendelian inheritance patterns of blast and BB resistance. Among the 50 best selected fixed advanced BC3F7 lines, 37 exhibited resistances conferred by all four genes. The aAdvanced lines (ALs) exhibited a significantly low disease score (0–1) for rice blast and BB compared to the BRRI dhan28 (7–9). Marker-trait association analysis showed molecular markers negatively correlated with disease susceptibility. The multi-location trial demonstrated the broad-spectrum resistance of the selected ALs against blast and BB with improved yield and quality traits. Among the ALs, BR(Path)13784-BC3-63–6-4-HR6 or G35 gave the maximum average yield (6.74 t ha−1) across the locations. The molecular markers used in this study can be a promising tool for candidate gene identification. Our advanced lines have the potential to be directly released as a commercial variety or used as a genetic stock in rice breeding programs to combine with other genes.