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First report of iris yellow spot virus infecting shallot (Allium cepa var. aggregatum) in Australia

  • Sari Nurulita,
  • Andrew D. W. Geering,
  • Kathleen S. Crew,
  • Stephen Harper,
  • John E. Thomas

摘要

In 2019, symptoms typical of infection by iris yellow spot virus (IYSV; family Tospoviridae, genus Orthotospovirus) were observed on shallot (A. cepa var. aggregatum) grown at the Gatton Research Facility, Department of Agriculture and Fisheries, Queensland. Initial testing by DAS-ELISA was positive for IYSV, and subsequent specific RT-PCR amplifying part of the RdRp (L-segment) and sequencing of amplicons confirmed the IYSV infection. This is the first record of IYSV infection of shallot in Australia.