Objectives <p>To determine the prevalence of anemia and iron, folate, and vitamin B12 deficiency in the adolescents of Yadadri-Bhuvanagiri district of Telangana.</p> Methods <p>A cross-sectional community-based study was conducted. Prevalence of anemia and micronutrient deficiencies were calculated and analyzed for age, sex, socio-economic status, diet, menstruation, and growth.</p> Results <p>The prevalence of anemia and deficiency of iron, vitamin B12 and folate was 34.1%, 29.2%, 77.4%, and 8.7%. Anemia and iron deficiency were higher in girls compared to boys (41.2% vs 20.9%,, P &lt; 0.001; 39.5% vs 9.8%, P &lt; 0.001) and girls in active menstruation (44.6% vs 12.1%; 42.3% vs 21.2%, P &lt; 0.001). Anemia was more in older girls (15–19&#xa0;years) compared to those aged 10–14&#xa0;years (50% vs 28.9%; P &lt; 0.001).</p> Conclusions <p>Anemia is a significant health problem for older girls. Vitamin B12 deficiency is prevalent in nearly three-fourths of adolescents.</p>