<p><i>Varroa destructor</i>, the most damaging pest of the western honey bee (<i>Apis mellifera</i>), arrived in Australia in June 2022. This parasitic mite weakens bees and spreads harmful viruses. We investigated Australian beekeepers’ trust and perceptions of biomechanical <i>Varroa</i> control methods to identify barriers to adoption. An online survey and in-person interviews were conducted with commercial, semi-commercial, and hobbyist beekeepers. Data were analysed using generalised linear models and content analysis. While many beekeepers expressed interest in non-chemical approaches, most relied on chemical treatments. Although 80% had heard of biomechanical options, few applied them due to concerns about time, practicality, and efficacy. Interviews revealed limited available information and context-specific challenges, including high reinfestation rates and co-infestation by small hive beetles. Our findings highlight the need for targeted education and support to promote sustainable <i>Varroa destructor</i> management and provide guidelines for future research aligned with commercial beekeepers’ needs.</p>