A method to measure the receptor-mediated Ca2+ transients in non-adherent THP-1 monocytes using gelatin methacryloyl for immobilization in a bath perfusion system
摘要
Background
Ca2+ transients are essential for cellular activity. They regulate various physiological functions via Ca2+ release from the endoplasmic reticulum and external Ca2+ influx in response to environmental stimuli. Cell adherence is necessary for Ca2+ imaging in physiological experiments using bath perfusion systems.
ObjectivesTherefore, non-adherent cells, such as THP-1 cells, are difficult to visualize using Ca2+ imaging despite their importance in vitro immune system studies.
ResultsHere, we provide an effective method to assess Ca2+ transients in gelatin methacryloyl (GelMA)-encapsulated THP-1 cells using a bath perfusion system.
ConclusionOur protocol can be used to assess Ca2+ transients in other non-adherent cells.