Long intergenic non-protein coding RNA 1436 accelerates cell proliferation, migration and adhesion properties of the MUM-2B cell line via microRNA-513a-5p/CUG Triplet repeat-binding protein 1 axis to activate the phosphatidylinositol 3-kinase/Akt signaling pathway

Long intergenic non-protein coding RNA 1436 (LINC01436) has been reported as a critical regulator of tumor occurrence, but the association between LINC01436 and choroidal melanoma (CM) has hardly been explored. The study was to uncover the precise association of LINC01436 with CM.

CM tissues and their corresponding adjacent normal tissues were collected, normal chorocytes UM96 and CM cell lines (C918, MUM-2B, OCM-1, MUM-2C) were cultured. LINC01436, microRNA (miR)-513a-5p, and CUG Triplet repeat-binding protein 1 (CELF1) expression in CM tissues and cells were detected. The screened MUM-2B cell line was transfected with LINC01436, miR-513a-5p or CELF1-related sequences or plasmids to explore their biological effects on cells with the involvement of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. The expressions of PI3K/Akt pathway-related protein were measured after transfection, same as the proliferation, cell cycle distribution, apoptosis, adhesion, migration, and invasion of MUM-2B cells. The binding of LINC01436 with miR-513a-5p and the targeting of miR-513a-5p with CELF1 were verified.

LINC01436 and CELF1 were upregulated in CM tissues and cells, while miR-513a-5p was reduced. LINC01436 adsorbed miR-513a-5p as a sponge, which targeted CELF1. Silenced LINC01436 suppressed the activation of PI3K/Akt pathway in MUM-2B cells as well as cell proliferation, adhesion, and metastasis. Meanwhile, elevated LINC01436 showed the opposite effect on MUM-2B cells. Restoring miR-513a-5p reversed the influence of augmented LINC01436 on MUM-2B cells. MiR-513a-5p restrained the activation of PI3K/Akt pathway and malignant behaviors of MUM-2B cells via targeting CELF1.

LINC01436 promotes cell proliferation, migration, and adhesion properties of the MUM-2B cell line via miR-513a-5p/CELF1 axis, which may be related to the PI3K/Akt pathway.