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<p>Citrus greening also known as huanglongbing (HLB) is devastating disease worldwide. Indian citriculture presently threatened by the spread of HLB disease throughout the country. Numerous rootstocks were used to combat HLB disease but none of them give fully resistance against HLB disease. Developing an alternative rootstock is good option for breeders to overcome this issue and developed hybrids will used for QTL mapping and MAS. Complementary progenitors within the citrus germplasm, particularly from <i>Citrus</i> and its close relative <i>Poncirus</i>, can be strategically selected to combine desirable traits. The development of hybrids that consistently express key dominant traits, regardless of parental heterozygosity, represents an effective approach for generating new candidate rootstocks with improved characteristics. Two rootstocks (Carrizo citrange and Swingle citrumelo) were used as male parents (tolerant) while three scions (Daisy tangerine, Mosambi strains <i>i.e</i>., M3, and M4) were used as female parents (Susceptible). A reliable protocol was developed for improved hybrid recovery from six cross combinations by integrating morphological traits and simple sequence repeat (SSR) markers, following in vitro embryo rescue performed at five different fruit drop intervals. The highest proportion of hybrid seedlings, based on the presence of multifoliate leaves, was obtained from embryos rescued between 81–95 DAP. Beyond this interval, the proportion of hybrids progressively declined with advancing embryo developmental stages, reaching the lowest levels at 111–125 DAP. Notably, SSR marker analysis identified a significantly higher proportion of zygotic seedlings (36.36%) compared to morphological evaluation (9.09%), underscoring the superior accuracy of molecular markers for hybrid identification.</p>

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Retrieval of interspecific hybrids through embryo rescue from dropped fruits and zygotic seedling identification using SSR markers in citrus

  • 期刊论文

  • 发表日期：

    2026年1月3日

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  • Popat Nanaso Gaikwad,
  • Gurupkar Singh Sidhu,
  • Jagveer Singh,
  • Yallaling Sanjay Chintale,
  • Harinder Singh Rattanpal

摘要

Citrus greening also known as huanglongbing (HLB) is devastating disease worldwide. Indian citriculture presently threatened by the spread of HLB disease throughout the country. Numerous rootstocks were used to combat HLB disease but none of them give fully resistance against HLB disease. Developing an alternative rootstock is good option for breeders to overcome this issue and developed hybrids will used for QTL mapping and MAS. Complementary progenitors within the citrus germplasm, particularly from Citrus and its close relative Poncirus, can be strategically selected to combine desirable traits. The development of hybrids that consistently express key dominant traits, regardless of parental heterozygosity, represents an effective approach for generating new candidate rootstocks with improved characteristics. Two rootstocks (Carrizo citrange and Swingle citrumelo) were used as male parents (tolerant) while three scions (Daisy tangerine, Mosambi strains i.e., M3, and M4) were used as female parents (Susceptible). A reliable protocol was developed for improved hybrid recovery from six cross combinations by integrating morphological traits and simple sequence repeat (SSR) markers, following in vitro embryo rescue performed at five different fruit drop intervals. The highest proportion of hybrid seedlings, based on the presence of multifoliate leaves, was obtained from embryos rescued between 81–95 DAP. Beyond this interval, the proportion of hybrids progressively declined with advancing embryo developmental stages, reaching the lowest levels at 111–125 DAP. Notably, SSR marker analysis identified a significantly higher proportion of zygotic seedlings (36.36%) compared to morphological evaluation (9.09%), underscoring the superior accuracy of molecular markers for hybrid identification.