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<p><i>Melia dubia</i> Cav. is a multipurpose tree species primarily used in the wood-based industries. Also, due to ease of propagation and wide adaptability, this species is highly suitable for agroforestry. Considering its economic and ecological values, its de novo whole-genome sequencing and annotation are necessary for the development of genomic tools in tree breeding. A draft genome of 246.5&#xa0;Mb was assembled at 35.34 × coverage and annotated with 37,014 non-redundant, protein-coding genes. A total of 111,256 genomic variants were identified, of which 95,389 were SNPs. Also, the genome consists of 2597 protein families. Moreover, 4.8&#xa0;Mb (2.33%) repeats and 690 tRNAs were identified in the <i>Melia dubia</i> genome. Additionally, the phylogenetic analysis of <i>Melia dubia</i> revealed that it is closely related to <i>M. azedarach</i> and <i>Khaya senegalensis</i> within the Meliaceae family. This genomic resource facilitates further investigations into molecular breeding, conservation genomics, and trait improvement. The proposed future work includes pangenomic analysis and CRISPR-based functional studies to increase the genetic application of <i>Melia dubia</i> in forestry and climate-resilient ecosystems.</p>

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The draft nuclear genome of Melia dubia Cav.: assembly, annotation, and resources for molecular breeding and conservation

  • 期刊论文

  • 发表日期：

    2025年11月19日

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  • Kunasekaran Rajarajan,
  • Ayyanadar Arunachalam,
  • Arun Kumar Handa

摘要

Melia dubia Cav. is a multipurpose tree species primarily used in the wood-based industries. Also, due to ease of propagation and wide adaptability, this species is highly suitable for agroforestry. Considering its economic and ecological values, its de novo whole-genome sequencing and annotation are necessary for the development of genomic tools in tree breeding. A draft genome of 246.5 Mb was assembled at 35.34 × coverage and annotated with 37,014 non-redundant, protein-coding genes. A total of 111,256 genomic variants were identified, of which 95,389 were SNPs. Also, the genome consists of 2597 protein families. Moreover, 4.8 Mb (2.33%) repeats and 690 tRNAs were identified in the Melia dubia genome. Additionally, the phylogenetic analysis of Melia dubia revealed that it is closely related to M. azedarach and Khaya senegalensis within the Meliaceae family. This genomic resource facilitates further investigations into molecular breeding, conservation genomics, and trait improvement. The proposed future work includes pangenomic analysis and CRISPR-based functional studies to increase the genetic application of Melia dubia in forestry and climate-resilient ecosystems.