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<p>Silver nanoparticles, useful in everyday life, exhibited adverse effects on the development of <i>Drosophila melanogaster.</i> We aimed to evaluate whether the silver nanoparticles induced developmental inhibition in <i>Drosophila</i> was associated with the inhibitions of some larval genes regulating insulin signaling that might also alter larval glucose metabolism. 5 Male and 5 female adult <i>Drosophila melanogaster</i> were exposed to dietary silver nanoparticles, 25–300&#xa0;mg/L. Age-specific developmental delays and body lengths of the third instar larvae of control and silver nanoparticle treatment groups were measured. cDNAs were synthesized from total larval RNAs. mRNA expressions of the larval genes <i>dilp2</i>, <i>dilp5</i>, <i>dInR</i> and <i>dAkt</i> were evaluated by quantitative real-time PCR. Compared to control, silver nanoparticles caused dose dependent significant delays in the initial appearance of third&#xa0;instar larvae, pupae and young adults of the fly. Larval body length and mRNA expression of larval genes were significantly and dose dependently reduced in treatment groups. Compared to control, dose dependent and significantly higher glucose concentration was observed in the silver nanoparticle treated larval hemolymph. As the fly and human possess substantial similarities in genome and insulin signaling, we assumed that similar human developmental and metabolic defects could be induced due the maternal exposures of these nanoparticles during pregnancy.</p>

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Silver nanoparticle-induced inhibition of some larval insulin signaling genes accompanied with developmental retardation and elevation of larval circulating glucose level in Drosophila melanogaster

  • 期刊论文

  • 发表日期：

    2025年11月6日

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  • Ashim Kumar Basak,
  • Tridip Chatterjee,
  • Swapan Kumar Ghosh

摘要

Silver nanoparticles, useful in everyday life, exhibited adverse effects on the development of Drosophila melanogaster. We aimed to evaluate whether the silver nanoparticles induced developmental inhibition in Drosophila was associated with the inhibitions of some larval genes regulating insulin signaling that might also alter larval glucose metabolism. 5 Male and 5 female adult Drosophila melanogaster were exposed to dietary silver nanoparticles, 25–300 mg/L. Age-specific developmental delays and body lengths of the third instar larvae of control and silver nanoparticle treatment groups were measured. cDNAs were synthesized from total larval RNAs. mRNA expressions of the larval genes dilp2, dilp5, dInR and dAkt were evaluated by quantitative real-time PCR. Compared to control, silver nanoparticles caused dose dependent significant delays in the initial appearance of third instar larvae, pupae and young adults of the fly. Larval body length and mRNA expression of larval genes were significantly and dose dependently reduced in treatment groups. Compared to control, dose dependent and significantly higher glucose concentration was observed in the silver nanoparticle treated larval hemolymph. As the fly and human possess substantial similarities in genome and insulin signaling, we assumed that similar human developmental and metabolic defects could be induced due the maternal exposures of these nanoparticles during pregnancy.