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<p><i>Withania</i> is majorly known by its two commercially significant species i.e., <i>W. somnifera</i> and <i>W. coagulans</i>. While both the species are medicinally important and producers of bioactive diterpene lactones, <i>W. somnifera</i> thrives well in the nature and <i>W. coagulans</i> has been enlisted as endangered. The present study demonstrated the differential responses of both the species of <i>Withania</i> against short-term UV-B exposure. UV-B-induced oxidative damage was more prominent in <i>W. coagulans</i> as compared to <i>W. somnifera</i>.Induction of antioxidative enzymes and protective flavonoids were found to be more in UV-B treated <i>W. somnifera</i> as compared to <i>W. coagulans</i>. Gene level validation of stress responses was performed through expression analysis of <i>UVR8</i> and <i>COP1</i> involved in UV-B signaling, <i>Bax-inhibitor-1</i> and <i>DAD1</i> involved in UV-B-mediated cell death pathway and <i>ruvB-like protein 1&amp; 2</i> involved in DNA repair pathway. All of them were shown to have greater expression in <i>W. somnifera</i> as compared to <i>W. coagulans</i> under UV-B treatments. Further, UV-B treatment for 3&#xa0;h caused 34% and 40% significant induction in withaferin A biosynthesis in <i>W. somnifera</i> and <i>W. coagulans</i> respectively. The results were supported by higher expression of two key biosynthetic pathway genes SQS and CAS. The results conclusively indicated the better stress management strategies in <i>W. somnifera</i> as compared to <i>W. coagulans</i> against similar UV-B exposure.</p>

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Unravelling the UV-B response in two Withania species: biochemical and molecular insights into W. somnifera and W. coagulans

  • 期刊论文

  • 发表日期：

    2025年10月31日

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  • Anjali Tiwari,
  • Saumya Tripathi,
  • Lakee Sharma,
  • Neha Pandey

摘要

Withania is majorly known by its two commercially significant species i.e., W. somnifera and W. coagulans. While both the species are medicinally important and producers of bioactive diterpene lactones, W. somnifera thrives well in the nature and W. coagulans has been enlisted as endangered. The present study demonstrated the differential responses of both the species of Withania against short-term UV-B exposure. UV-B-induced oxidative damage was more prominent in W. coagulans as compared to W. somnifera.Induction of antioxidative enzymes and protective flavonoids were found to be more in UV-B treated W. somnifera as compared to W. coagulans. Gene level validation of stress responses was performed through expression analysis of UVR8 and COP1 involved in UV-B signaling, Bax-inhibitor-1 and DAD1 involved in UV-B-mediated cell death pathway and ruvB-like protein 1& 2 involved in DNA repair pathway. All of them were shown to have greater expression in W. somnifera as compared to W. coagulans under UV-B treatments. Further, UV-B treatment for 3 h caused 34% and 40% significant induction in withaferin A biosynthesis in W. somnifera and W. coagulans respectively. The results were supported by higher expression of two key biosynthetic pathway genes SQS and CAS. The results conclusively indicated the better stress management strategies in W. somnifera as compared to W. coagulans against similar UV-B exposure.