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<p>Harnessing heterosis through F<sub>1</sub> hybrids is one of the most efficient strategies for enhancing productivity of crops including bitter gourd. However, the development of heterotic hybrids relies (among others) on combining genetically diverse inbred parents to create breeding populations (BPs) to derive promising new inbred lines. In this context, the present study aimed to (i) assess the genetic diversity among advanced breeding lines (ABLs) and parents of elite single cross hybrids (SCHs) using simple sequence repeat (SSR) markers and (ii) identify the most desirable inbred line combinations for BP development, (iii) explore efficiency of SSR marker alleles to discriminate ABLs for their fruit yield potential and (iv) identify SSR marker alleles unique to ABLs. Twenty-five lines which included 18 elite advanced breeding lines (ABLs) and seven parents of four commercialised elite SCHs—Mayura, Aachi, OSBT-03 and Piccalo were genotyped using 100 SSR markers. The results revealed polymorphism of 45 markers. The number of alleles per SSR locus ranged from 2 to 7. Narrow difference between average (2.36) and effective (1.71) number of alleles per locus suggest near-equal frequency of alleles detected at each polymorphic locus. The 35 markers with three or more alleles and PIC values of ≥ 0.5 proved to be more informative than those with two alleles. The SSR marker alleles were efficient to discriminate ABLs for their fruit yield potential. The seven pairs of ABLs with greater dissimilarity index (≥ 0.35) were identified (which also differed for fruit yield plant<sup>−1</sup>, fruit length and fruit diameter) for use in developing BPs to recover superior inbred lines better than those used in the study.</p> Graphical abstract <p></p>

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Richness of SSR marker alleles and their efficiency to discriminate bitter gourd (Momordica charantia L.) advanced breeding lines for their fruit yielding potential

  • 期刊论文

  • 发表日期：

    2025年10月29日

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  • Balthikumeru Vishwanatha Sinchana,
  • Sampangi Ramesh,
  • Chindi Basavaraj Siddu,
  • Muniraja Pallavi,
  • Gonal Basanagouda,
  • Mugali Pundalik Kalpana,
  • Dinesh Sindhu,
  • Saikia Kuldeep,
  • Annabathula Mohan Rao,
  • Vataparavi Sangappa Channabasava,
  • Bisalere Puttappa Maruthi Prasad,
  • Venkatesha Prashantha,
  • Sobanada Prakash Prem Sagar

摘要

Harnessing heterosis through F1 hybrids is one of the most efficient strategies for enhancing productivity of crops including bitter gourd. However, the development of heterotic hybrids relies (among others) on combining genetically diverse inbred parents to create breeding populations (BPs) to derive promising new inbred lines. In this context, the present study aimed to (i) assess the genetic diversity among advanced breeding lines (ABLs) and parents of elite single cross hybrids (SCHs) using simple sequence repeat (SSR) markers and (ii) identify the most desirable inbred line combinations for BP development, (iii) explore efficiency of SSR marker alleles to discriminate ABLs for their fruit yield potential and (iv) identify SSR marker alleles unique to ABLs. Twenty-five lines which included 18 elite advanced breeding lines (ABLs) and seven parents of four commercialised elite SCHs—Mayura, Aachi, OSBT-03 and Piccalo were genotyped using 100 SSR markers. The results revealed polymorphism of 45 markers. The number of alleles per SSR locus ranged from 2 to 7. Narrow difference between average (2.36) and effective (1.71) number of alleles per locus suggest near-equal frequency of alleles detected at each polymorphic locus. The 35 markers with three or more alleles and PIC values of ≥ 0.5 proved to be more informative than those with two alleles. The SSR marker alleles were efficient to discriminate ABLs for their fruit yield potential. The seven pairs of ABLs with greater dissimilarity index (≥ 0.35) were identified (which also differed for fruit yield plant−1, fruit length and fruit diameter) for use in developing BPs to recover superior inbred lines better than those used in the study.

Graphical abstract