Scholarlink

<p><i>Helopeltis theivora</i> Waterhouse (Heteroptera: Miridae) is one of the most important sucking pests of tea responsible for substantial yield loss. Tea-growing regions such as Upper Assam (Up_Assam), South Bank (SB_Assam), North Bank (NB_Assam), South Bank (Insecticide Non_Exposed), Central Dooars (CD_WB) and Darjeeling (Dar_WB) were selected for the collection of <i>H. theivora</i> populations in order to examine insecticide resistance and susceptibility against three insecticides namely thiamethoxam, deltamethrin and quinalphos. The majority of populations showed high to moderate resistance to thiamethoxam and deltamethrin, except two, Dar_WB and Non_Exposed. Notably, in all populations, there was no resistance for quinalphos. Additionally, elevated level of detoxifying enzymes like general esterase, cytochrome P450 monooxygenase and glutathione S-transferase was found in resistant populations in comparison to susceptible ones. Increased resistance was correlated with higher enzyme expression that suggested the existence of insecticide detoxification mechanisms in them. According to the phylogenetic analysis based on ISSR markers derived from twenty primers, the susceptible Dar_WB showed significant divergence from the rest of the populations. The susceptible Non_Exposed from Assam was found to be phylogenetically similar to resistant populations, signifying the possible epigenetic regulation for resistance to insecticides. These results identify the dynamicity of insecticide resistance phenomenon and provide the insect genotype resources for further molecular characterization and resistance specific marker identification.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Understanding genotypic diversity of tea mosquito bug, Helopeltis theivora Waterhouse (Heteroptera: Miridae) populations identified for insecticide susceptibility and resistance

  • 期刊论文

  • 发表日期：

    2025年2月20日

查看全文
  • Sudipta Naskar,
  • Anjan Hazra,
  • Sanatan Ghosh,
  • Rupjyoti Das,
  • Azariah Babu,
  • Somnath Roy,
  • Rituparna Kundu Chaudhuri,
  • Dipankar Chakraborti

摘要

Helopeltis theivora Waterhouse (Heteroptera: Miridae) is one of the most important sucking pests of tea responsible for substantial yield loss. Tea-growing regions such as Upper Assam (Up_Assam), South Bank (SB_Assam), North Bank (NB_Assam), South Bank (Insecticide Non_Exposed), Central Dooars (CD_WB) and Darjeeling (Dar_WB) were selected for the collection of H. theivora populations in order to examine insecticide resistance and susceptibility against three insecticides namely thiamethoxam, deltamethrin and quinalphos. The majority of populations showed high to moderate resistance to thiamethoxam and deltamethrin, except two, Dar_WB and Non_Exposed. Notably, in all populations, there was no resistance for quinalphos. Additionally, elevated level of detoxifying enzymes like general esterase, cytochrome P450 monooxygenase and glutathione S-transferase was found in resistant populations in comparison to susceptible ones. Increased resistance was correlated with higher enzyme expression that suggested the existence of insecticide detoxification mechanisms in them. According to the phylogenetic analysis based on ISSR markers derived from twenty primers, the susceptible Dar_WB showed significant divergence from the rest of the populations. The susceptible Non_Exposed from Assam was found to be phylogenetically similar to resistant populations, signifying the possible epigenetic regulation for resistance to insecticides. These results identify the dynamicity of insecticide resistance phenomenon and provide the insect genotype resources for further molecular characterization and resistance specific marker identification.