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<p>The endemic genus&#xa0;<i>Eutyphoeus</i> (Crassiclitellata: Acanthodrilidae) plays a significant role in temperate and subtropical regions of India and was collected from Mizoram state of the Northeastern Region (NER). This work aims to assess the diversity of the genus based on the integrative morpho-anatomical features and sequencing of the mitochondrial&#xa0;cytochrome oxidase subunit 1 (CO1) gene, study phylogenetic relationships among species, and evaluate their genetic variability at the regional scale. Our morpho-anatomical results revealed six&#xa0;<i>Eutyphoeus</i> species namely&#xa0;<i>E. comillahnus, E. festivus, E. gigas, E. mizoramensis, E. pharpingianus,</i> and <i>E. scutarius</i> respectively out of which <i>E. comillahnus, E. festivus</i>,&#xa0;<i>E. scutarius,</i> and<i>, E. pharpingianus</i> are new earthworm records from Mizoram. Additionally,&#xa0;<i>E. pharpingianus</i> was reported for the first time from the entire NER. Based on the Kimura 2 parameter model we observed 3.08% mean intraspecific and 12.35% minimum interspecific genetic distances in species, and the species delimitation methods show a consensus of a clear barcode gap of 7–10%. The phylogenetic relationships based on the Maximum Likelihood (ML) tree revealed three well-supported major clades reflecting the potential of CO1 in earthworms. However, more sampling and multi-loci data are required for in-depth analysis. In addition, the network analysis and genetic variability parameters such as haplotype diversity (hd) and nucleotide diversity (π) are provided for each species. Such information can be used for studying their population structure, biogeography, and dispersal which have a substantial effect on biodiversity and conservation owing to perturbances and anthropogenic activities.</p>

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Barcoding and genetic variation in Eutyphoeus species (Crassiclitellata: Acanthodrilidae) with first report of E. pharpingianus (Michaelsen 1907) from Mizoram, North Eastern Region (NER) of India

  • 期刊论文

  • 发表日期：

    2025年2月12日

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  • Azhar Rashid Lone,
  • Samrendra Singh Thakur,
  • Pooja Tiwari,
  • Samuel Wooster James,
  • Shweta Yadav

摘要

The endemic genus Eutyphoeus (Crassiclitellata: Acanthodrilidae) plays a significant role in temperate and subtropical regions of India and was collected from Mizoram state of the Northeastern Region (NER). This work aims to assess the diversity of the genus based on the integrative morpho-anatomical features and sequencing of the mitochondrial cytochrome oxidase subunit 1 (CO1) gene, study phylogenetic relationships among species, and evaluate their genetic variability at the regional scale. Our morpho-anatomical results revealed six Eutyphoeus species namely E. comillahnus, E. festivus, E. gigas, E. mizoramensis, E. pharpingianus, and E. scutarius respectively out of which E. comillahnus, E. festivus, E. scutarius, and, E. pharpingianus are new earthworm records from Mizoram. Additionally, E. pharpingianus was reported for the first time from the entire NER. Based on the Kimura 2 parameter model we observed 3.08% mean intraspecific and 12.35% minimum interspecific genetic distances in species, and the species delimitation methods show a consensus of a clear barcode gap of 7–10%. The phylogenetic relationships based on the Maximum Likelihood (ML) tree revealed three well-supported major clades reflecting the potential of CO1 in earthworms. However, more sampling and multi-loci data are required for in-depth analysis. In addition, the network analysis and genetic variability parameters such as haplotype diversity (hd) and nucleotide diversity (π) are provided for each species. Such information can be used for studying their population structure, biogeography, and dispersal which have a substantial effect on biodiversity and conservation owing to perturbances and anthropogenic activities.