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<p>Breast cancer (BC) is thought to be the main cause of cancer-related deaths in women. One biomarker with reported heterogeneity in BC is HER2, a component of the <i>EGF</i> receptor (EGFR) family. Traditionally, HER2-positive breast cancer has been associated with a worse prognosis and inferior outcomes in survival. In the field of oncology, the introduction of phytochemicals or nutraceuticals has shown promising outcomes in terms of disease prevention, treatment, and/or harnessing. In the present work, the in vitro anti-cancer effect of the <i>Quercus brantii</i> gall (QBG) extract and Tannic acid (TA) as the most important component of the extract was evaluated using the MTT assay, quantitative real-time polymerase chain reaction (RT-PCR) and flow-cytometry assay on SKBR3 and MDA-MB-468 BC cells and compared with Tamoxifen (TAM). The MTT assay indicated that QBG significantly inhibited the proliferation rate of both cancer cell lines at the concentration of 500&#xa0;µg/ml. In SKBR3 cells, QBG significantly decreased both HER2 and EGFR expression. TA with TAM combination therapy represented a significant decrease. In MDA-MB-468 cells, QBG, TA, or TAM could not effectively reduce EGFR expression alone, but the combination of TA with TAM significantly reduced EGFR expression. The flow-cytometry assay also verified the anti-cancer effect of QBG with its inducing apoptosis effect on SKBR3 and MDA-MB-468 cancer cells. Overall, these findings revealed that QBG has a cytotoxicity effect on SKBR3 and MDA-MB-468 cells via inducing apoptosis, but the sharp decrease in HER2 levels in the RT-PCR test indicated that QBG has a stronger anti-cancer effect on SKBR3 cells.</p>

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Quercus brantii gall alcoholic extract induces apoptosis by suppressing EGFR/HER2 signaling in human breast cancer cells

  • 期刊论文

  • 发表日期：

    2025年3月5日

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  • Mohamad Shourmij,
  • Parvaneh Najafizadeh,
  • Jaber Zafari,
  • Javad Khalili Fard,
  • Zahra Mousavi

摘要

Breast cancer (BC) is thought to be the main cause of cancer-related deaths in women. One biomarker with reported heterogeneity in BC is HER2, a component of the EGF receptor (EGFR) family. Traditionally, HER2-positive breast cancer has been associated with a worse prognosis and inferior outcomes in survival. In the field of oncology, the introduction of phytochemicals or nutraceuticals has shown promising outcomes in terms of disease prevention, treatment, and/or harnessing. In the present work, the in vitro anti-cancer effect of the Quercus brantii gall (QBG) extract and Tannic acid (TA) as the most important component of the extract was evaluated using the MTT assay, quantitative real-time polymerase chain reaction (RT-PCR) and flow-cytometry assay on SKBR3 and MDA-MB-468 BC cells and compared with Tamoxifen (TAM). The MTT assay indicated that QBG significantly inhibited the proliferation rate of both cancer cell lines at the concentration of 500 µg/ml. In SKBR3 cells, QBG significantly decreased both HER2 and EGFR expression. TA with TAM combination therapy represented a significant decrease. In MDA-MB-468 cells, QBG, TA, or TAM could not effectively reduce EGFR expression alone, but the combination of TA with TAM significantly reduced EGFR expression. The flow-cytometry assay also verified the anti-cancer effect of QBG with its inducing apoptosis effect on SKBR3 and MDA-MB-468 cancer cells. Overall, these findings revealed that QBG has a cytotoxicity effect on SKBR3 and MDA-MB-468 cells via inducing apoptosis, but the sharp decrease in HER2 levels in the RT-PCR test indicated that QBG has a stronger anti-cancer effect on SKBR3 cells.