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<p>To facilitate the prompt diagnosis of SARS-CoV-2 infection, a sandwich immunocolloidal gold chromatography assay targeting the envelope proteins of SARS-CoV-2 was developed. BALB/c mice were immunized with purified recombinant SARS-CoV-2 E protein. Positive hybridoma cells were generated through standard fusion techniques, and the corresponding monoclonal antibodies (mAbs) were isolated from ascites fluid using the caprylic acid–ammonium sulfate precipitation method. Subsequently, a double-antibody sandwich immunocolloidal gold chromatography system was constructed for diagnostic application. The sensitivity, specificity, and concordance rate of the methodology were rigorously assessed.</p>

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Establishment and Evaluation of Immunocolloidal Gold Chromatography Method in the Sandwich Mode for Novel Coronavirus Double-Antibody Detection

  • 期刊论文

  • 发表日期：

    2025年6月3日

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  • Hai Li,
  • Han Wu,
  • Changyan Yang,
  • Jiayi Shu,
  • Jianglong Wang,
  • Haitao Li,
  • Hefeng Zhou,
  • Min Shao

摘要

To facilitate the prompt diagnosis of SARS-CoV-2 infection, a sandwich immunocolloidal gold chromatography assay targeting the envelope proteins of SARS-CoV-2 was developed. BALB/c mice were immunized with purified recombinant SARS-CoV-2 E protein. Positive hybridoma cells were generated through standard fusion techniques, and the corresponding monoclonal antibodies (mAbs) were isolated from ascites fluid using the caprylic acid–ammonium sulfate precipitation method. Subsequently, a double-antibody sandwich immunocolloidal gold chromatography system was constructed for diagnostic application. The sensitivity, specificity, and concordance rate of the methodology were rigorously assessed.