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<p>The resurrection plant <i>Selaginella bryopteris</i> (Selaginellaceae), commonly known as ‘Sanjeevani’, exhibits remarkable desiccation tolerance and rapid recovery upon rehydration. This study investigated the physiological responses of the resurrection plant <i>S. bryopteris</i> during a complete desiccation–rehydration–desiccation cycle using relative water content, chlorophyll content, chlorophyll fluorescence, and JIP-test analyses. Physiological responses were evaluated using five biological replicates and three technical replicates, with statistical significance at <i>p</i> &lt; 0.05. During rehydration, RWC increased from nearly 0 to 85% within 30&#xa0;h, accompanied by a 214% increase in chlorophyll content. Recovery of photosynthetic activity was evidenced by approximately 10-fold and 11-fold increases in maximum fluorescence (Fm) and active reaction centre density (RC/CS), respectively. Similarly, the maximum quantum yield of primary photochemistry (φP<sub>0</sub>) and electron transport efficiency (φE<sub>0</sub>) increased by about 800% and 775%, indicating rapid restoration of PSII functionality and electron transport capacity. Performance index (PIcs) increased by more than 1700%, reflecting coordinated recovery of the photosynthetic apparatus. During subsequent dehydration, photosynthetic parameters remained relatively stable during the initial phase of water loss but declined sharply thereafter. RC/CS, φP<sub>0</sub>, φE<sub>0</sub>, and PIcs decreased progressively, whereas energy dissipation parameters (DI<sub>0</sub>/RC and φD<sub>0</sub>) increased, indicating a shift from photochemical energy utilization to thermal energy dissipation. OJIP analysis revealed that recovery was both sequential and biphasic, with rapid restoration of PSII photochemistry preceding slower recovery of downstream electron transport processes. The findings indicate that reversible PSII downregulation, preservation of reaction-centre functionality, and controlled energy dissipation collectively contribute to desiccation tolerance in <i>S. bryopteris</i>.</p>

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A comprehensive analysis of photosynthetic energy flux dynamics in the resurrection plant Selaginella bryopteris during desiccation–rehydration and rehydration–desiccation transitions

  • 期刊论文

  • 发表日期：

    2026年7月8日

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  • Upma Bhatt,
  • Yashwant Sompura,
  • Shubhangani Sharma,
  • Kavya Dashora,
  • Reto J. Strasser,
  • Vineet Soni

摘要

The resurrection plant Selaginella bryopteris (Selaginellaceae), commonly known as ‘Sanjeevani’, exhibits remarkable desiccation tolerance and rapid recovery upon rehydration. This study investigated the physiological responses of the resurrection plant S. bryopteris during a complete desiccation–rehydration–desiccation cycle using relative water content, chlorophyll content, chlorophyll fluorescence, and JIP-test analyses. Physiological responses were evaluated using five biological replicates and three technical replicates, with statistical significance at p < 0.05. During rehydration, RWC increased from nearly 0 to 85% within 30 h, accompanied by a 214% increase in chlorophyll content. Recovery of photosynthetic activity was evidenced by approximately 10-fold and 11-fold increases in maximum fluorescence (Fm) and active reaction centre density (RC/CS), respectively. Similarly, the maximum quantum yield of primary photochemistry (φP0) and electron transport efficiency (φE0) increased by about 800% and 775%, indicating rapid restoration of PSII functionality and electron transport capacity. Performance index (PIcs) increased by more than 1700%, reflecting coordinated recovery of the photosynthetic apparatus. During subsequent dehydration, photosynthetic parameters remained relatively stable during the initial phase of water loss but declined sharply thereafter. RC/CS, φP0, φE0, and PIcs decreased progressively, whereas energy dissipation parameters (DI0/RC and φD0) increased, indicating a shift from photochemical energy utilization to thermal energy dissipation. OJIP analysis revealed that recovery was both sequential and biphasic, with rapid restoration of PSII photochemistry preceding slower recovery of downstream electron transport processes. The findings indicate that reversible PSII downregulation, preservation of reaction-centre functionality, and controlled energy dissipation collectively contribute to desiccation tolerance in S. bryopteris.