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<p>Current research aims to investigate a less explored bacterium <i>V. fluvialis</i> VB1 from biggest natural carbon dioxide sink marine origin in carbon sequestration study. Carbonic anhydrase (CA) producing bacteria was isolated from marine origin. Out of 35 bacterial isolates, three bacterial (VB1, VB2 and VB3) isolates showed CO<sub>2</sub> fixing ability in primary screening test. Among which one of the isolates, VB1 showed high esterase activity in secondary screening. The isolate VB1 was taken further for identification, purification of CA and further experimental findings. The CA strain was identified as <i>Vibrio fluvialis</i> by 16&#xa0;S-rRNA sequence analysis and sequence submitted in NCBI-GenBank. Furthermore, for more enzyme titres optimization of bacterial growth, a response surface methodology (RSM) was employed by Box-Benken designs (BBDs) model. The optimized parameter resulted in elevation of enzyme production led to their activity from 121.15 µmol/ml to 138.56 µmol/ml. Moreover, the biosequestration activity of CA showed positive result by bioprecipitation of CO<sub>2</sub> into CaCO<sub>3</sub> confirmed by SEM and EDAX. By which <i>V. fluvialis</i> can be added up on list of potential strain for carbon sequestration and can be used to establish sustainable CCUs.</p>

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Biosequestration of carbon dioxide by a key enzyme carbonic anhydrase (CA) of Vibrio fluvialis VB1 from marine origin: notion for sustainable era

  • 期刊论文

  • 发表日期：

    2026年6月15日

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  • Priya Gupta,
  • Nalini Soni,
  • Silambarasan Tamil Selvan,
  • Chinnaperumal Kamaraj,
  • Balasubramanian Velramar

摘要

Current research aims to investigate a less explored bacterium V. fluvialis VB1 from biggest natural carbon dioxide sink marine origin in carbon sequestration study. Carbonic anhydrase (CA) producing bacteria was isolated from marine origin. Out of 35 bacterial isolates, three bacterial (VB1, VB2 and VB3) isolates showed CO2 fixing ability in primary screening test. Among which one of the isolates, VB1 showed high esterase activity in secondary screening. The isolate VB1 was taken further for identification, purification of CA and further experimental findings. The CA strain was identified as Vibrio fluvialis by 16 S-rRNA sequence analysis and sequence submitted in NCBI-GenBank. Furthermore, for more enzyme titres optimization of bacterial growth, a response surface methodology (RSM) was employed by Box-Benken designs (BBDs) model. The optimized parameter resulted in elevation of enzyme production led to their activity from 121.15 µmol/ml to 138.56 µmol/ml. Moreover, the biosequestration activity of CA showed positive result by bioprecipitation of CO2 into CaCO3 confirmed by SEM and EDAX. By which V. fluvialis can be added up on list of potential strain for carbon sequestration and can be used to establish sustainable CCUs.