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<p>A detailed comparative genomic analysis was conducted on eight <i>Methanocaldococcus</i> strains, focusing on their phylogeny, metabolic pathways, adaptive traits and biotechnological potential. Functional attributes consists of conserved pathway of methane metabolism alongside variable gene clusters associated with the sulfur and nitrogen cycling. Phylogenomic analyses using Average Nucleotide Identity (ANI), Genome to Genome Distance Calculator (GGDC) and EasyCGTree revealed high genetic variability across the genus, with <i>M. infernus</i> ME and <i>M. indicus</i> SL43 being the most divergent members. CRISPR-Cas and phage analyses showed that the strains consist of a substantial number of CRISPR arrays with diverse spacer sequences. Protein-protein interaction networks were constructed on the methane, suphur and nitrogen metabolic pathway proteins, which highlighted MtrA and Mer proteins as hub-bottleneck proteins of the network. This in-silico study of the genus <i>Methanocaldococcus</i> provides insights that inform future experimental investigations into archaeal metabolism, evolutionary adaptation and extremophile biology.</p>

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Comparative genomic and network analysis of Methanocaldococcus genus reveals genetic diversity and adaptive metabolic traits

  • 期刊论文

  • 发表日期：

    2026年6月15日

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  • Anfas Muhammed,
  • Janhvi Tripathi,
  • Helianthous Verma

摘要

A detailed comparative genomic analysis was conducted on eight Methanocaldococcus strains, focusing on their phylogeny, metabolic pathways, adaptive traits and biotechnological potential. Functional attributes consists of conserved pathway of methane metabolism alongside variable gene clusters associated with the sulfur and nitrogen cycling. Phylogenomic analyses using Average Nucleotide Identity (ANI), Genome to Genome Distance Calculator (GGDC) and EasyCGTree revealed high genetic variability across the genus, with M. infernus ME and M. indicus SL43 being the most divergent members. CRISPR-Cas and phage analyses showed that the strains consist of a substantial number of CRISPR arrays with diverse spacer sequences. Protein-protein interaction networks were constructed on the methane, suphur and nitrogen metabolic pathway proteins, which highlighted MtrA and Mer proteins as hub-bottleneck proteins of the network. This in-silico study of the genus Methanocaldococcus provides insights that inform future experimental investigations into archaeal metabolism, evolutionary adaptation and extremophile biology.