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<p>Twenty endophytic bacterial strains isolated from <i>Cycas revoluta</i> coralloid roots were characterized for their salt stress tolerance and phyto-beneficial properties, mainly indole-3-acetic acid production, siderophore secretion and phosphate solubilization under both optimal and stressful conditions. Their genetic diversity was assessed through 16&#xa0;S rRNA gene sequencing. Results demonstrated a significant diversity of genera, including <i>Agrobacterium</i>, <i>Pseudomonas</i>, <i>Pantoea</i>, <i>Bacillus</i>, <i>Peribacillus</i>, <i>Ochrobactrum</i>, and <i>Stenotrophomonas</i>. Salt tolerance assays and PGP screening revealed the high tolerance threshold and PGP potential of two of isolated bacteria (<i>Pantoea</i> sp. LMR-C62 and <i>Bacillus</i> sp. LMR-C11). Tomato (<i>Solanum lycopersicum</i> L. cv Campbell 33) seeds bio-priming with these strains significantly improved germination rate (up to 75.3% at 150 mM NaCl) and decreased the mean germination time compared to non-inoculated seeds. In greenhouse trials, plantlet inoculation with these two strains led to significant improvements in root length (up to +55%), shoot dry weight (+72%), leaf area (+33%), and relative water content (+92%) under salt stress conditions. Indeed, tomato inoculation improved root development, leaf area, and plant height. At the physiological level, the results indicated positive effects of the inoculation on chlorophyll and anthocyanin contents. These findings demonstrate that <i>Cycas revoluta</i>-associated bacterial endophytes, particularly <i>Panteoa</i> sp. LMR-C62 and <i>Bacillus</i> sp. LMR-C11, hold robust potential as biofertilizer candidates for enhancing tomato growth and resilience under saline conditions. To our knowledge, this is the first report prospecting the unique coralloid root microbiome of <i>Cycas revoluta</i> for agricultural purposes, revealing novel halotolerant endophytes with notable biofertilizer potential.</p>

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Bioprospecting Cycas revoluta-associated bacterial endophytes for tomato (Solanum lycopersicum L.) growth promotion under salt stress

  • 期刊论文

  • 发表日期：

    2026年6月2日

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  • Jalila Benjelloun,
  • Mohamed Hnini,
  • Sarah Bouzroud,
  • Imane El Attar,
  • Najoua Mghazli,
  • Asma Hami,
  • Chouhra Talbi,
  • Jamal Aurag,
  • Abdelaziz Smouni,
  • Abdelkarim Guedira,
  • Kaoutar Taha

摘要

Twenty endophytic bacterial strains isolated from Cycas revoluta coralloid roots were characterized for their salt stress tolerance and phyto-beneficial properties, mainly indole-3-acetic acid production, siderophore secretion and phosphate solubilization under both optimal and stressful conditions. Their genetic diversity was assessed through 16 S rRNA gene sequencing. Results demonstrated a significant diversity of genera, including Agrobacterium, Pseudomonas, Pantoea, Bacillus, Peribacillus, Ochrobactrum, and Stenotrophomonas. Salt tolerance assays and PGP screening revealed the high tolerance threshold and PGP potential of two of isolated bacteria (Pantoea sp. LMR-C62 and Bacillus sp. LMR-C11). Tomato (Solanum lycopersicum L. cv Campbell 33) seeds bio-priming with these strains significantly improved germination rate (up to 75.3% at 150 mM NaCl) and decreased the mean germination time compared to non-inoculated seeds. In greenhouse trials, plantlet inoculation with these two strains led to significant improvements in root length (up to +55%), shoot dry weight (+72%), leaf area (+33%), and relative water content (+92%) under salt stress conditions. Indeed, tomato inoculation improved root development, leaf area, and plant height. At the physiological level, the results indicated positive effects of the inoculation on chlorophyll and anthocyanin contents. These findings demonstrate that Cycas revoluta-associated bacterial endophytes, particularly Panteoa sp. LMR-C62 and Bacillus sp. LMR-C11, hold robust potential as biofertilizer candidates for enhancing tomato growth and resilience under saline conditions. To our knowledge, this is the first report prospecting the unique coralloid root microbiome of Cycas revoluta for agricultural purposes, revealing novel halotolerant endophytes with notable biofertilizer potential.