Scholarlink

<p>Xylooligosaccharides (XOS) were produced from xylan-rich agricultural residues using endo-xylanase from <i>Thermomyces lanuginosus</i> PC7S1T immobilized on magnetic chitosan beads. The immobilized enzyme showed high immobilization yield (90.7%), efficiency (73.7%), and activity recovery (66.8%). Enhanced stability was observed, with 94% residual activity after 50 d at 4&#xa0;°C and 54% after 10 reuse cycles, along with improved thermal (70–75&#xa0;°C) and pH stability (pH 6.5). Immobilization altered the product profile, favoring xylotriose (X3) and xylotetraose (X4), whereas the free enzyme predominantly produced xylobiose (X2), highlighting its potential for prebiotic applications. Scanning electron microscopy confirmed effective substrate depolymerization. Toxicity assays using <i>Artemia salina</i> showed no significant differences in survival between XOS produced by free and immobilized enzymes (<i>p</i> &gt; 0.05), indicating low acute toxicity. Overall, these results reveal the potential of this immobilized system for sustainable and scalable production of prebiotic XOS from renewable biomass.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Immobilization of endo-xylanase from Thermomyces lanuginosus PC7S1T for selective production of xylooligosaccharides from agricultural residues

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月21日

查看全文
  • Laura Camila Wagner Rodio,
  • Maria Eduarda Carboniéri,
  • Tatiane Sayuri Inagaki,
  • Diandra de Andrades,
  • Maria de Lourdes Teixeira de Moraes Polizeli,
  • Paula Daniela Helfenstein Rother,
  • Marco Antônio Záchia Ayub,
  • José Luis da Conceição Silva,
  • Alexandre Maller,
  • Thaís Duarte Bifano,
  • Rita de Cássia Garcia Simão,
  • Marina Kimiko Kadowaki

摘要

Xylooligosaccharides (XOS) were produced from xylan-rich agricultural residues using endo-xylanase from Thermomyces lanuginosus PC7S1T immobilized on magnetic chitosan beads. The immobilized enzyme showed high immobilization yield (90.7%), efficiency (73.7%), and activity recovery (66.8%). Enhanced stability was observed, with 94% residual activity after 50 d at 4 °C and 54% after 10 reuse cycles, along with improved thermal (70–75 °C) and pH stability (pH 6.5). Immobilization altered the product profile, favoring xylotriose (X3) and xylotetraose (X4), whereas the free enzyme predominantly produced xylobiose (X2), highlighting its potential for prebiotic applications. Scanning electron microscopy confirmed effective substrate depolymerization. Toxicity assays using Artemia salina showed no significant differences in survival between XOS produced by free and immobilized enzymes (p > 0.05), indicating low acute toxicity. Overall, these results reveal the potential of this immobilized system for sustainable and scalable production of prebiotic XOS from renewable biomass.