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<p>This research was developed a neuron specific enolase (NSE) biosensor on titanium oxide (TiO<sub>2</sub>) modified interdigitated microelectrodes (IDME). Biotin-streptavidin strategy was utilized to immobilize the aptamer on IDME. It was identified that higher number of streptavidin and aptamer complex captured on IDME when the surface modified with TiO<sub>2</sub> compared with a direct immobilization. The sandwich pattern with aptamer and antibody was performed on TiO<sub>2</sub>-modified surfaces. The detection limit of NSE was identified as 0.1&#xa0;ng/mL with an R<sup>2</sup> value of 0.9826 [y = 9.6984x—13.089]. To enhance the detection performance, the antibody was conjugated with goldurchin and it increases the current flow, detecting NSE-spiked serum as low as 0.1&#xa0;ng/mL. Besides, control experiments with Interleukin-6, Carcinoembryonic antigen, and complementary aptamer sequence (a non-binding aptamer) fail to enhance the current responses. This NSE biosensor quantifies the lower level of target and helps to diagnose lung cancer and severity.</p>

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Quantifying neuron-specific enolase on TiO2-goldurchin-modified electrodes for lung cancer diagnosis

  • 期刊论文

  • 发表日期：

    2026年5月20日

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  • Xueying Yang,
  • Thangavel Lakshmipriya,
  • Subash C.B. Gopinath,
  • Ying Li

摘要

This research was developed a neuron specific enolase (NSE) biosensor on titanium oxide (TiO2) modified interdigitated microelectrodes (IDME). Biotin-streptavidin strategy was utilized to immobilize the aptamer on IDME. It was identified that higher number of streptavidin and aptamer complex captured on IDME when the surface modified with TiO2 compared with a direct immobilization. The sandwich pattern with aptamer and antibody was performed on TiO2-modified surfaces. The detection limit of NSE was identified as 0.1 ng/mL with an R2 value of 0.9826 [y = 9.6984x—13.089]. To enhance the detection performance, the antibody was conjugated with goldurchin and it increases the current flow, detecting NSE-spiked serum as low as 0.1 ng/mL. Besides, control experiments with Interleukin-6, Carcinoembryonic antigen, and complementary aptamer sequence (a non-binding aptamer) fail to enhance the current responses. This NSE biosensor quantifies the lower level of target and helps to diagnose lung cancer and severity.