Scholarlink

<p>Tramadol has been classified as a prohibited substance in several countries due to a significant rise in overdoses and related deaths over the past decade. Black barberry (BB) has demonstrated therapeutic potential in various conditions, including metabolic, neurodegenerative, and cardiovascular disorders. This study investigated the protective effects of BB against tramadol-induced cognitive and neural impairments. Thirty-six rats were divided into three groups: control, tramadol (50&#xa0;mg/kg daily for 21 days with a standard diet), and Tramadol + BB (same tramadol dose plus a diet containing 4% BB for 21 days). Behavioral tests—including the Y-maze, tail suspension, elevated plus maze, and Morris water maze—were conducted. Electrophysiological recordings, immunohistochemistry for GFAP, stereological analysis, neuronal spatial arrangement, and Western blotting were also performed. The BB-supplemented diet significantly improved memory performance in the Tramadol + BB group compared to tramadol-only rats. Field potential analysis showed recovery of the fEPSP slope to near-control levels. Western blot results indicated increased expression of brain-derived neurotrophic factor (BDNF) and repressor element 1-silencing transcription factor (REST) in the hippocampus. BB also reduced tramadol-induced astrogliosis, although astrocyte morphology was largely unchanged except for a decrease in branch number. Additionally, BB enhanced glutathione (GSH) levels and reduced reactive oxygen species (ROS). These findings suggest that BB mitigates tramadol-induced neurotoxicity, potentially through its antioxidant properties and modulation of REST and BDNF pathways. Further research is needed to clarify the molecular mechanisms underlying these protective effects.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The black barberry diet decreases oxidative stress and improves behavioral functions in adult male rats induced tramadol neurodegeneration: behavioral, molecular and histopathological studies

  • 期刊论文

  • 发表日期：

    2026年4月25日

查看全文
  • Azam Sajjadi Nasab,
  • Amir Masoud Dalband,
  • Ibrahim Mohammadzadeh,
  • Zahrasadat Lajevardi,
  • Maral Moafi,
  • Mohammad Javad Ebrahimi,
  • Seyedeh Naghmeh Nourirad,
  • Alireza Asadi,
  • Arsham Efazati,
  • Amirreza Beirami,
  • Maral Hasanzadeh,
  • Mohammad Nikoohemmat,
  • Ali Asadi Zeidabadi,
  • Armin Alinezhad,
  • Fatemeh Zolfaghari,
  • Ramtin Hajibeygi,
  • Kimia Vakili,
  • Amir-Hossein Bayat,
  • Siavash Parvardeh,
  • Reza Mastery Farahani,
  • Hamzeh Badeli Sarkala,
  • Mojtaba Sani,
  • Meysam Hassani Moghaddam,
  • Abbas Aliaghaei

摘要

Tramadol has been classified as a prohibited substance in several countries due to a significant rise in overdoses and related deaths over the past decade. Black barberry (BB) has demonstrated therapeutic potential in various conditions, including metabolic, neurodegenerative, and cardiovascular disorders. This study investigated the protective effects of BB against tramadol-induced cognitive and neural impairments. Thirty-six rats were divided into three groups: control, tramadol (50 mg/kg daily for 21 days with a standard diet), and Tramadol + BB (same tramadol dose plus a diet containing 4% BB for 21 days). Behavioral tests—including the Y-maze, tail suspension, elevated plus maze, and Morris water maze—were conducted. Electrophysiological recordings, immunohistochemistry for GFAP, stereological analysis, neuronal spatial arrangement, and Western blotting were also performed. The BB-supplemented diet significantly improved memory performance in the Tramadol + BB group compared to tramadol-only rats. Field potential analysis showed recovery of the fEPSP slope to near-control levels. Western blot results indicated increased expression of brain-derived neurotrophic factor (BDNF) and repressor element 1-silencing transcription factor (REST) in the hippocampus. BB also reduced tramadol-induced astrogliosis, although astrocyte morphology was largely unchanged except for a decrease in branch number. Additionally, BB enhanced glutathione (GSH) levels and reduced reactive oxygen species (ROS). These findings suggest that BB mitigates tramadol-induced neurotoxicity, potentially through its antioxidant properties and modulation of REST and BDNF pathways. Further research is needed to clarify the molecular mechanisms underlying these protective effects.