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<p>Recent advancements in biotechnology have opened new avenues for enhancing both the qualitative and quantitative production of medicinal plants. This review highlights various genetic modification approaches, including transgenesis, recombinant DNA technology (RDT), and gene amplification through polymerase chain reaction (PCR), which enable the targeted improvement of plant traits. Complementary techniques such as omics technologies and RNA interference (RNAi) further allow precise regulation of gene expression, elucidation of metabolic pathway and enhancement of metabolite biosynthesis (e.g., alkaloids, terpenoids). In addition, this review discusses innovative strategies to improve plant yield and quality, including genetic marker-based identification, cryopreservation for long-term conservation, plant tissue culture for large-scale propagation, nanotechnology-based delivery systems, bio-fortification, DNA barcoding, and metabolomics for sustainable utilization. The integration of these biotechnological tools signficantly contributes to the production of high-value bioactive compounds, secondary metabolites, and recombinant proteins, while also facilitating drug discovery pipelines. Moreover, these interventions enhance tolerance to environmental stresses, promoting adaptation under adverse conditions.</p>

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A comprehensive review on biotechnological approaches for improvement of medicinal plants: techniques for qualitative and quantitative production

  • 期刊论文

  • 发表日期：

    2025年11月22日

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  • Jagriti Kamra,
  • Pradyumn Saini,
  • Om Prakash,
  • Vivek Kumar,
  • Rudramani Tiwari

摘要

Recent advancements in biotechnology have opened new avenues for enhancing both the qualitative and quantitative production of medicinal plants. This review highlights various genetic modification approaches, including transgenesis, recombinant DNA technology (RDT), and gene amplification through polymerase chain reaction (PCR), which enable the targeted improvement of plant traits. Complementary techniques such as omics technologies and RNA interference (RNAi) further allow precise regulation of gene expression, elucidation of metabolic pathway and enhancement of metabolite biosynthesis (e.g., alkaloids, terpenoids). In addition, this review discusses innovative strategies to improve plant yield and quality, including genetic marker-based identification, cryopreservation for long-term conservation, plant tissue culture for large-scale propagation, nanotechnology-based delivery systems, bio-fortification, DNA barcoding, and metabolomics for sustainable utilization. The integration of these biotechnological tools signficantly contributes to the production of high-value bioactive compounds, secondary metabolites, and recombinant proteins, while also facilitating drug discovery pipelines. Moreover, these interventions enhance tolerance to environmental stresses, promoting adaptation under adverse conditions.