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<p>Isolation and evaluation of phytostimulatory properties of bacterial endophytes were performed from <i>Rhododendron griffithianum</i> Wight from Himalayan region of Darjeeling, India. Two potent endophytes were identified as <i>Kocuria rhizophila</i> RGDS06 and <i>Pantoea vagans</i> RGDS02 by 16&#xa0;S rRNA gene sequencing analysis. Indole-3-acetic acid (IAA) was purified and confirmed by HPLC from two potent IAA producers RGDS06 and RGDS02. <i>K. rhizophila</i> RGDS06 (224.41 ± 1.11&#xa0;µg/mL) and <i>P. vagans</i> RGDS02 (202.4 ± 1.24&#xa0;µg/mL) were highest IAA producers. Genes involved in key regulatory steps of the IAA biosynthetic pathway, such as the <i>nit</i> gene from <i>K. rhizophila</i> RGDS06 and the <i>ipd</i>C gene from <i>P. vagans</i> RGDS02, were amplified using End-Point PCR method. Two endophytes aided in the growth of <i>Oryza sativa</i> var. Shatabdi and <i>Vigna radiata</i> var. Virat. Physiological parameters like root and shoot length, as well as fresh and dry weight, of both monocot and dicot seedlings increased in the presence of <i>K. rhizophila</i> RGDS06 and <i>P. vagans</i> RGDS02. Chlorophyll content was also enhanced in endophyte-treated seedlings compared to control seedlings. RT-PCR revealed upregulated expression of <i>OsPIN</i>1a, <i>OsPIN</i>1b, <i>OsPIN</i>1c, and <i>OsARF</i>1 in bioprimed <i>O. sativa</i>, and <i>VrPIN</i>1, <i>VrARF</i> genes in <i>V. radiata</i> roots, compared to control seedlings. The growth of tested fungal phytopathogens <i>Pestalotiopsis</i> species and <i>Phytophthora</i> species was suppressed by <i>K. rhizophila</i> RGDS06. But, <i>P. vagans</i> RGDS02 showed antagonistic effect only against <i>Pestalotiopsis</i> species. The mycelial distortion of tested pathogens was studied by FESEM. This study demonstrates the potential of these bacterial endophytes as bioinoculants and biocontrol agents.</p>

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Gene expression analysis in Oryza sativa and Vigna radiata primed with Kocuria rhizophila and Pantoea vagans from Himalayan rhododendrons, India

  • 期刊论文

  • 发表日期：

    2025年11月22日

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  • Shreyasi Biswas,
  • Rajeshwari Chatterjee,
  • Soumi Guha Polley,
  • Mahuya Mukhopadhyay

摘要

Isolation and evaluation of phytostimulatory properties of bacterial endophytes were performed from Rhododendron griffithianum Wight from Himalayan region of Darjeeling, India. Two potent endophytes were identified as Kocuria rhizophila RGDS06 and Pantoea vagans RGDS02 by 16 S rRNA gene sequencing analysis. Indole-3-acetic acid (IAA) was purified and confirmed by HPLC from two potent IAA producers RGDS06 and RGDS02. K. rhizophila RGDS06 (224.41 ± 1.11 µg/mL) and P. vagans RGDS02 (202.4 ± 1.24 µg/mL) were highest IAA producers. Genes involved in key regulatory steps of the IAA biosynthetic pathway, such as the nit gene from K. rhizophila RGDS06 and the ipdC gene from P. vagans RGDS02, were amplified using End-Point PCR method. Two endophytes aided in the growth of Oryza sativa var. Shatabdi and Vigna radiata var. Virat. Physiological parameters like root and shoot length, as well as fresh and dry weight, of both monocot and dicot seedlings increased in the presence of K. rhizophila RGDS06 and P. vagans RGDS02. Chlorophyll content was also enhanced in endophyte-treated seedlings compared to control seedlings. RT-PCR revealed upregulated expression of OsPIN1a, OsPIN1b, OsPIN1c, and OsARF1 in bioprimed O. sativa, and VrPIN1, VrARF genes in V. radiata roots, compared to control seedlings. The growth of tested fungal phytopathogens Pestalotiopsis species and Phytophthora species was suppressed by K. rhizophila RGDS06. But, P. vagans RGDS02 showed antagonistic effect only against Pestalotiopsis species. The mycelial distortion of tested pathogens was studied by FESEM. This study demonstrates the potential of these bacterial endophytes as bioinoculants and biocontrol agents.