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<p>Marine-derived chitosan is a valuable biomaterial due to its biocompatibility and functional properties. In this work, chitosan extracted from the cuttlebone of <i>Sepia pharaonis</i> was used to prepare silver–chitosan nanoparticles (Ag-Chi-NPs) by a chemical reduction process. The synthesis was confirmed by comprehensive characterization: UV–Vis spectra displayed a plasmon resonance peak at 420&#xa0;nm, FTIR identified interactions between amino and hydroxyl groups, XRD revealed crystalline silver phases, and FESEM showed spherical nanoparticles with a size range of 15–40&#xa0;nm. Following these analyses, the Ag-Chi-NPs exhibited significant bioactivity. Antioxidant activity was substantial, with hydrogen peroxide scavenging of 84.5% (IC₅₀ 18.09&#xa0;µg/mL), DPPH radical inhibition of 65.6% (IC₅₀ 29.04&#xa0;µg/mL), and a total antioxidant capacity of 55.5% at 100&#xa0;µg/mL. Antimicrobial assays further demonstrated inhibition zones of 21 ± 0.6&#xa0;mm for <i>Streptococcus mutans</i>, 19 ± 0.2&#xa0;mm for&#xa0;<i>Staphylococcus aureus</i>, and 14 ± 0.1&#xa0;mm for <i>Escherichia coli</i>, while <i>Candida albicans</i> showed no susceptibility. Cytotoxicity testing using KB-1 carcinoma cells indicated excellent biocompatibility, with cell viability above 95% at lower concentrations and concentration-dependent inhibition at 200&#xa0;µg/mL. Overall, these results highlight Ag-Chi-NPs as sustainable bio-nanocomposites that combine antioxidant, antibacterial, and cytocompatibility properties, making them promising candidates for wound healing materials, antimicrobial coatings, and potential therapeutic applications.</p>

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Silver nanochitosan: a sustainable approach for enhanced antimicrobial, antioxidant, and anticancer applications

  • 期刊论文

  • 发表日期：

    2025年9月14日

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  • Saranya Elumalai,
  • Senthil Nathan Madeshwaran,
  • Ambiga Somasundaram,
  • Pasiyappazham Ramasamy

摘要

Marine-derived chitosan is a valuable biomaterial due to its biocompatibility and functional properties. In this work, chitosan extracted from the cuttlebone of Sepia pharaonis was used to prepare silver–chitosan nanoparticles (Ag-Chi-NPs) by a chemical reduction process. The synthesis was confirmed by comprehensive characterization: UV–Vis spectra displayed a plasmon resonance peak at 420 nm, FTIR identified interactions between amino and hydroxyl groups, XRD revealed crystalline silver phases, and FESEM showed spherical nanoparticles with a size range of 15–40 nm. Following these analyses, the Ag-Chi-NPs exhibited significant bioactivity. Antioxidant activity was substantial, with hydrogen peroxide scavenging of 84.5% (IC₅₀ 18.09 µg/mL), DPPH radical inhibition of 65.6% (IC₅₀ 29.04 µg/mL), and a total antioxidant capacity of 55.5% at 100 µg/mL. Antimicrobial assays further demonstrated inhibition zones of 21 ± 0.6 mm for Streptococcus mutans, 19 ± 0.2 mm for Staphylococcus aureus, and 14 ± 0.1 mm for Escherichia coli, while Candida albicans showed no susceptibility. Cytotoxicity testing using KB-1 carcinoma cells indicated excellent biocompatibility, with cell viability above 95% at lower concentrations and concentration-dependent inhibition at 200 µg/mL. Overall, these results highlight Ag-Chi-NPs as sustainable bio-nanocomposites that combine antioxidant, antibacterial, and cytocompatibility properties, making them promising candidates for wound healing materials, antimicrobial coatings, and potential therapeutic applications.