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<p>The coastal area of Thailand is a tropical marine environment with high microbial diversity, providing favorable conditions for microorganisms capable of degrading bioplastics. The current study aimed to investigate the bacterial community profiling of four samples collected from a coastal area in Thailand and to isolate the potential thermophilic bacteria with the ability to produce bioplastic-degrading enzymes. Our analysis revealed site-specific predominant genera: <i>Brevibacillus</i> in seawater (64.34 ± 0.27%), <i>Pseudomonas</i> in plastic waste (39.69 ± 3.77%), <i>Pseudoalteromonas</i> in soil (54.83 ± 2.40%), and <i>Psychrobacter</i> in moss rock (41.01 ± 1.67%). The thermophilic bacteria, including 6 poly(lactic acid) (PLA)- and 3 poly(butylene succinate) (PBS)-degrading bacteria, were isolated using a two-step technique in an emulsified polymer medium. These nine isolates were classified into five species across four genera: <i>Brevibacillus gelatini</i>, <i>Microbispora rosea</i>, <i>Actinomadura keratinilytica</i>, <i>Paenibacillus thermoaerophilus</i>, and <i>P. ginsengihumi</i>. Among these, <i>Actinomadura keratinilytica</i> LDF1 and <i>M. rosea</i> BS2-4 exhibited the highest enzymatic activities for PLA and PBS degradation (0.87 ± 0.11&#xa0;U/mL and 0.31 ± 0.03&#xa0;U/mL, respectively). Scanning electron microscopy confirmed the degradation capabilities of these strains in culture medium. Crude enzyme from the LDF1 strain demonstrated versatility in degrading various types of PLA, including PLA film, PLA powder, commercial cup, and commercial cutlery, while the strain BS2-4 enzyme effectively degraded PBS in film, powder, commercial cup, and commercial drinking straw. These findings advance our understanding of coastal microbial ecology and also highlight the potential of indigenous bacteria for bioplastic waste management, contributing to sustainable environmental solutions.</p>

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Profiling of the bacterial community and the degradative capability of newly isolated poly(lactic acid) (PLA)- and poly(butylene succinate) (PBS)-degrading bacteria from coastal samples

  • 期刊论文

  • 发表日期：

    2025年9月17日

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  • Ausawadee Phonlamai,
  • Wanthanee Khetkorn,
  • Voranuch Thongpool,
  • Titiporn Panyachanakul,
  • Chanwit Suriyachadkun,
  • Vichien Kitpreechavanich,
  • Chatsuda Sakdapetsiri,
  • Thanasak Lomthong

摘要

The coastal area of Thailand is a tropical marine environment with high microbial diversity, providing favorable conditions for microorganisms capable of degrading bioplastics. The current study aimed to investigate the bacterial community profiling of four samples collected from a coastal area in Thailand and to isolate the potential thermophilic bacteria with the ability to produce bioplastic-degrading enzymes. Our analysis revealed site-specific predominant genera: Brevibacillus in seawater (64.34 ± 0.27%), Pseudomonas in plastic waste (39.69 ± 3.77%), Pseudoalteromonas in soil (54.83 ± 2.40%), and Psychrobacter in moss rock (41.01 ± 1.67%). The thermophilic bacteria, including 6 poly(lactic acid) (PLA)- and 3 poly(butylene succinate) (PBS)-degrading bacteria, were isolated using a two-step technique in an emulsified polymer medium. These nine isolates were classified into five species across four genera: Brevibacillus gelatini, Microbispora rosea, Actinomadura keratinilytica, Paenibacillus thermoaerophilus, and P. ginsengihumi. Among these, Actinomadura keratinilytica LDF1 and M. rosea BS2-4 exhibited the highest enzymatic activities for PLA and PBS degradation (0.87 ± 0.11 U/mL and 0.31 ± 0.03 U/mL, respectively). Scanning electron microscopy confirmed the degradation capabilities of these strains in culture medium. Crude enzyme from the LDF1 strain demonstrated versatility in degrading various types of PLA, including PLA film, PLA powder, commercial cup, and commercial cutlery, while the strain BS2-4 enzyme effectively degraded PBS in film, powder, commercial cup, and commercial drinking straw. These findings advance our understanding of coastal microbial ecology and also highlight the potential of indigenous bacteria for bioplastic waste management, contributing to sustainable environmental solutions.