Scholarlink

<p>In this study, we describe the adjuvanticity of biogenic AuNPs, previously synthesized as 40–50&#xa0;nm spherical particles from cell-free fermentation broth of <i>Streptomyces</i> sp. M137-2. Bovine serum albumin (BSA), a model antigen, was conjugated to biogenic AuNPs to form AuNPs–BSA. The conjugates were characterize using UV–Vis spectroscopy, scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FT-IR). Both biogenic AuNPs and AuNPs–BSA showed high viability (over 70%) in macrophages derived from U937 cells. Biogenic AuNPs enhanced the uptake of conjugated BSA by macrophages. AuNPs–BSA-stimulated macrophages exhibited cytokine levels of TNF-α and IL-6 that were 4.5 times (<i>p</i> &lt; 0.0001) and 4.6 times (<i>p</i> = 0.004) higher, respectively, as compared to BSA-stimulated cells. The adjuvanticity of the biogenic AuNPs is likely attributed to their antigen-carrier properties, which enhance antigen uptake by macrophages, as well as their immunostimulatory properties that induce strong pro-inflammatory responses. This study represents the first proposal of biogenic AuNPs as an adjuvant candidate, providing further studies in in vivo models.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Biogenic gold nanoparticles synthesized using Streptomyces sp. M137-2 as potential vaccine adjuvant

  • 期刊论文

  • 发表日期：

    2025年7月7日

查看全文
  • Nefise Ünlüer,
  • Aytül Gül-Mete,
  • Elif Esin Hameş

摘要

In this study, we describe the adjuvanticity of biogenic AuNPs, previously synthesized as 40–50 nm spherical particles from cell-free fermentation broth of Streptomyces sp. M137-2. Bovine serum albumin (BSA), a model antigen, was conjugated to biogenic AuNPs to form AuNPs–BSA. The conjugates were characterize using UV–Vis spectroscopy, scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FT-IR). Both biogenic AuNPs and AuNPs–BSA showed high viability (over 70%) in macrophages derived from U937 cells. Biogenic AuNPs enhanced the uptake of conjugated BSA by macrophages. AuNPs–BSA-stimulated macrophages exhibited cytokine levels of TNF-α and IL-6 that were 4.5 times (p < 0.0001) and 4.6 times (p = 0.004) higher, respectively, as compared to BSA-stimulated cells. The adjuvanticity of the biogenic AuNPs is likely attributed to their antigen-carrier properties, which enhance antigen uptake by macrophages, as well as their immunostimulatory properties that induce strong pro-inflammatory responses. This study represents the first proposal of biogenic AuNPs as an adjuvant candidate, providing further studies in in vivo models.