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<p>Vinblastine, a potent anticancer drug, is traditionally extracted from&#xa0;<i>Catharanthus roseus</i>, but its low yield and high production costs necessitate alternative sources. In this study, twelve endophytic fungi were isolated from&#xa0;<i>Catharanthus roseus</i>&#xa0;collected in Himachal Pradesh, India. Among these, isolate VPF-2, identified as&#xa0;<i>Alternaria alternata</i>&#xa0;through morphological and molecular analysis, demonstrated significant vinblastine production (8.673&#xa0;mg/L) in M3 medium broth. PCR screening confirmed the presence of key biosynthetic genes, desacetoxyvindoline-4-hydroxylase (D4H) and tryptophan decarboxylase (TDC), in VPF-2. The fungal-derived vinblastine exhibited cytotoxic activity against MDCK cancer cells, with an IC<sub>50</sub> value of 69.03&#xa0;µg/mL. Structural characterization using HPTLC, UV, FTIR, LC–ESI–MS/MS, and NMR confirmed the compound's identity. This study reports the highest vinblastine yield from&#xa0;<i>A. alternata</i>&#xa0;to date, highlighting its potential as a sustainable and scalable alternative for industrial vinblastine production.</p>

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Sustainable vinblastine production by Alternaria alternata, an endophytic fungus isolated from Catharanthus roseus in the Northern Himalayan Region

  • 期刊论文

  • 发表日期：

    2025年5月26日

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  • Aditya Banyal,
  • Rahul Thakur,
  • Pryanka Thakur,
  • Vikram Thakur,
  • Duni Chand,
  • Pradeep Kumar

摘要

Vinblastine, a potent anticancer drug, is traditionally extracted from Catharanthus roseus, but its low yield and high production costs necessitate alternative sources. In this study, twelve endophytic fungi were isolated from Catharanthus roseus collected in Himachal Pradesh, India. Among these, isolate VPF-2, identified as Alternaria alternata through morphological and molecular analysis, demonstrated significant vinblastine production (8.673 mg/L) in M3 medium broth. PCR screening confirmed the presence of key biosynthetic genes, desacetoxyvindoline-4-hydroxylase (D4H) and tryptophan decarboxylase (TDC), in VPF-2. The fungal-derived vinblastine exhibited cytotoxic activity against MDCK cancer cells, with an IC50 value of 69.03 µg/mL. Structural characterization using HPTLC, UV, FTIR, LC–ESI–MS/MS, and NMR confirmed the compound's identity. This study reports the highest vinblastine yield from A. alternata to date, highlighting its potential as a sustainable and scalable alternative for industrial vinblastine production.