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<p>Okra (<i>Abelmoschus esculentus</i> L.) is a major vegetable crop with significant commercial value, which suffers from yield losses due to various biotic stresses. Begomoviruses (family <i>Geminiviridae</i>) cause severe infection in okra in warmer regions worldwide. In 2023, a severe disease infection was observed in okra (<i>Ablemoschus esculentus</i> L.) at the research farm of ICAR–Indian Agriculture Research Institute, New Delhi, India. In this study a novel alphasatellite associated with the monopartite okra enation leaf curl virus (OELCuV) (<i>Begomovirus abelsmoschusenation</i>) begomovirus was identified by rolling circle amplification (RCA) in north India. Sequence analysis of OELCuV showed 88.98–99.02% nucleotide&#xa0;(nt) sequence identity with other OELCuV isolates. The alphasatellite shared 85.11% and 87.73% nt sequence identity with the symptomless alphasatellite associated with hollyhock yellow vein virus&#xa0;isolate Lahore 2 (NC_039084.1) and hollyhock yellow vein alphasatellite isolate J47 (MF683829.1), respectively. According to the species demarcation&#xa0;criteria based on the&#xa0;sequence similarity, it was concluded that it is a novel alphasatellite associated with OELCuV.</p>

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Identification and molecular characterization of a novel satellite associated with okra enation leaf curl virus infected okra (Abelmoschus esculentus L.) in India

  • 期刊论文

  • 发表日期：

    2025年5月16日

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  • Nishant,
  • Ramesh Kumar Yadav,
  • Damini Diksha,
  • Malyaj R. Prajapati,
  • Harshwardhan Choudhary,
  • Suman Lata,
  • Mailem Y. Shimray,
  • Reshav Naik,
  • Nitika Gupta,
  • Virendra Kumar Baranwal,
  • Susheel Kumar Sharma

摘要

Okra (Abelmoschus esculentus L.) is a major vegetable crop with significant commercial value, which suffers from yield losses due to various biotic stresses. Begomoviruses (family Geminiviridae) cause severe infection in okra in warmer regions worldwide. In 2023, a severe disease infection was observed in okra (Ablemoschus esculentus L.) at the research farm of ICAR–Indian Agriculture Research Institute, New Delhi, India. In this study a novel alphasatellite associated with the monopartite okra enation leaf curl virus (OELCuV) (Begomovirus abelsmoschusenation) begomovirus was identified by rolling circle amplification (RCA) in north India. Sequence analysis of OELCuV showed 88.98–99.02% nucleotide (nt) sequence identity with other OELCuV isolates. The alphasatellite shared 85.11% and 87.73% nt sequence identity with the symptomless alphasatellite associated with hollyhock yellow vein virus isolate Lahore 2 (NC_039084.1) and hollyhock yellow vein alphasatellite isolate J47 (MF683829.1), respectively. According to the species demarcation criteria based on the sequence similarity, it was concluded that it is a novel alphasatellite associated with OELCuV.