<p>The need for new environmentally friendly materials has become more critical than ever to prevent the acceleration of environmental degradation driven by global population growth. Biopolymer derivatives—renewable resources widely used in nature and in numerous human applications—have attracted growing interest due to their abundance, recyclability, and potential to replace conventional chemical plastics. In this study, we report the fabrication of periodic dielectric templates using soft lithography on pure corn-starch polyglucan films (with defined amylose and amylopectin content). Subsequent metallization of these sub-micrometer patterned films produces plasmonic structures supporting surface plasmon resonance (SPR) applications. This approach provides a simple and repeatable method for generating structural color on dielectric biopolymers, a property typically arising from crystalline and amorphous lamellae formed by amylose double helices and branched amylopectin segments. However, this study focuses only on SPR peaks via UV–Vis spectrum comparisons. The starch-based periodic dielectric templates exhibit environmentally friendly characteristics and, depending on the biopolymer derivative used, may be either water-soluble or water-insoluble. Starch films templated into various submicrometer sizes display tunable structural colors and can be engineered as biodegradable biopolymer-based timers. Furthermore, the plasmonic interface can enhance the photoluminescence of metal surfaces or organic dye–containing layers. We also demonstrate the fabrication of plasmonic arrays with precise optical properties via metallization of these periodic dielectric templates.</p>