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<p>Plant breeding can influence the bacterial community in the rhizosphere. However, the effect of plant breeding on plant growth-promoting rhizobacteria (PGPR) remains unclear. This study aimed to assess the PGPR community in the rhizosphere of parental and segregating generations during lima bean (<i>Phaseolus lunatus</i> L.) breeding. Four lima bean genotypes were evaluated: P<sub>1</sub> (UFPI 628; originating from Brazil), P<sub>2</sub> (G25276; originating from Argentina), F<sub>2</sub> (a more segregating generation), and F<sub>7</sub> (a less segregating generation). The PGPR community in the rhizosphere was analyzed using 16&#xa0;S rRNA sequencing. The PGPR community in the rhizosphere of P<sub>2</sub> differed from those of P<sub>1</sub>, F<sub>2</sub>, and F<sub>7</sub>. The community in P<sub>1</sub> was similar to that of F<sub>2</sub>, while F<sub>7</sub> clustered separately. Enrichment of PGPR taxa showed <i>Cupriavidus</i> and <i>Actinoplanes</i> in P<sub>1</sub>, whereas P<sub>2</sub> was enriched with <i>Pausteria</i> and <i>Microbacterium</i>. The rhizosphere of F<sub>2</sub> showed enrichment of <i>Rhodopseudomonas</i> and <i>Dactylosporangium</i>, while F<sub>7</sub> was enriched with <i>Paenibacillus</i> and <i>Azospirillum</i>. As breeding progressed, the complexity of PGPR interactions changed, with F<sub>7</sub> showing the lowest complexity but the highest number of positive interactions. The proportion of specialist PGPR increased in F<sub>7</sub> compared to P<sub>1</sub> but decreased compared to P<sub>2</sub>. This study demonstrates that lima bean breeding significantly affects the PGPR community, with segregating generations showing reduced complexity but increased positive interactions, suggesting enhanced beneficial cooperation.</p>

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Lima bean breeding changes the community of plant growth-promoting rhizobacteria in the rhizosphere

  • 期刊论文

  • 发表日期：

    2025年4月10日

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  • Karla Annielle da Silva Bernardo Brito,
  • Sandra Mara Barbosa Rocha,
  • Ângela Celis de Almeida Lopes,
  • Romario Martins Costa,
  • Sabrina Hermelindo Ventura,
  • Erica Maria Batista Araujo,
  • João Vitor Morais Sousa,
  • Regina Lucia Ferreira Gomes,
  • Arthur Prudêncio de Araujo Pereira,
  • Erika Valente de Medeiros,
  • Lucas William Mendes,
  • Ana Roberta Lima de Miranda,
  • Ademir Sergio Ferreira Araujo

摘要

Plant breeding can influence the bacterial community in the rhizosphere. However, the effect of plant breeding on plant growth-promoting rhizobacteria (PGPR) remains unclear. This study aimed to assess the PGPR community in the rhizosphere of parental and segregating generations during lima bean (Phaseolus lunatus L.) breeding. Four lima bean genotypes were evaluated: P1 (UFPI 628; originating from Brazil), P2 (G25276; originating from Argentina), F2 (a more segregating generation), and F7 (a less segregating generation). The PGPR community in the rhizosphere was analyzed using 16 S rRNA sequencing. The PGPR community in the rhizosphere of P2 differed from those of P1, F2, and F7. The community in P1 was similar to that of F2, while F7 clustered separately. Enrichment of PGPR taxa showed Cupriavidus and Actinoplanes in P1, whereas P2 was enriched with Pausteria and Microbacterium. The rhizosphere of F2 showed enrichment of Rhodopseudomonas and Dactylosporangium, while F7 was enriched with Paenibacillus and Azospirillum. As breeding progressed, the complexity of PGPR interactions changed, with F7 showing the lowest complexity but the highest number of positive interactions. The proportion of specialist PGPR increased in F7 compared to P1 but decreased compared to P2. This study demonstrates that lima bean breeding significantly affects the PGPR community, with segregating generations showing reduced complexity but increased positive interactions, suggesting enhanced beneficial cooperation.