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<p>Addressing the challenges of rigid cell wall structure, low polysaccharide yield, and unclear anti-ulcerative colitis (UC) potential in <i>Ganoderma lucidum</i> fruiting bodies, this study innovatively employed a green synergistic extraction method combining hydroxyl radicals (generated from H<sub>2</sub>O<sub>2</sub> within the system) and natural deep eutectic solvents (NADES). After screening the optimal NADES (choline chloride-urea, 1:2 molar ratio), extraction parameters were optimized via single-factor experiments and Box-Behnken design, achieving a 35.60% ± 0.37% yield of GLPs-HD—11.16-fold higher than traditional hot-water extraction (GLPs-WE). In a dextran sulfate sodium (DSS)-induced UC mouse model, both GLPs-HD and GLPs-WE exhibited comparable protective effects: effectively mitigating weight loss, preserving colon length, reducing LPS translocation, enhancing antioxidant enzyme activities (SOD, GSH-Px), lowering ALT levels, and significantly suppressing pro-inflammatory cytokines (TNF-α, IL-6) (<i>p</i> &lt; 0.05). Notably, the novel extraction method not only improved yield but also maintained the biological activity of polysaccharides. These findings provide robust scientific evidence for the efficient preparation of <i>G. lucidum</i> polysaccharides and the development of related functional products for UC management.</p> Graphical abstract <p></p>

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Synergistic and efficient extraction of the polysaccharides Ganoderma lucidum using hydroxyl radical-deep eutectic solvent and its intervention effect on ulcerative colitis in mice

  • 期刊论文

  • 发表日期：

    2026年6月19日

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  • Yingxuan Gu,
  • Han Shu,
  • Fen Huang,
  • Xuyang Liu,
  • Yi Ji,
  • Jiale Liang,
  • Lijun Chen,
  • Hongcheng Zhao,
  • Yike Wu,
  • Zixun Huang,
  • Liyun Zhu,
  • Yongjun Zhang

摘要

Addressing the challenges of rigid cell wall structure, low polysaccharide yield, and unclear anti-ulcerative colitis (UC) potential in Ganoderma lucidum fruiting bodies, this study innovatively employed a green synergistic extraction method combining hydroxyl radicals (generated from H2O2 within the system) and natural deep eutectic solvents (NADES). After screening the optimal NADES (choline chloride-urea, 1:2 molar ratio), extraction parameters were optimized via single-factor experiments and Box-Behnken design, achieving a 35.60% ± 0.37% yield of GLPs-HD—11.16-fold higher than traditional hot-water extraction (GLPs-WE). In a dextran sulfate sodium (DSS)-induced UC mouse model, both GLPs-HD and GLPs-WE exhibited comparable protective effects: effectively mitigating weight loss, preserving colon length, reducing LPS translocation, enhancing antioxidant enzyme activities (SOD, GSH-Px), lowering ALT levels, and significantly suppressing pro-inflammatory cytokines (TNF-α, IL-6) (p < 0.05). Notably, the novel extraction method not only improved yield but also maintained the biological activity of polysaccharides. These findings provide robust scientific evidence for the efficient preparation of G. lucidum polysaccharides and the development of related functional products for UC management.

Graphical abstract