<p>Flavonoids are commonly employed as inhibitors of amylases. This study evaluated the inhibitory effects of rutin on both α-amylase and α-glucosidase, as well as its impact on the starch digestibility in corn starch-lauric acid (CS-LA) complexes with different levels of amylose. Rutin exhibited a stronger inhibitory effect on α-glucosidase than α-amylase, with mixed-type inhibition, while α-amylase inhibition was competitive. In CS-LA complexes, rutin significantly increased resistance to starch hydrolysis in waxy corn starch (WCS) and normal corn starch (NCS) complexes. However, it did not weaken the regulation effect on the digestibility of high-amylose corn starch (HACS)-LA complexes. The results demonstrated that the impact of rutin on starch hydrolysis in CS-LA complexes was influenced by the amylose content. This study enhances comprehension of the interplay between flavonoids and starch digestibility in the complexes with different amylose content, thereby aiding the development of starchy foods with reduced digestibility.</p>