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<p>This work developed an ultrasound-assisted enzymatic extraction (UAEE) method for producing <i>Rana chensinensis</i> ovum oil (RCOO), a valuable source of omega-3 and omega-6 fatty acids. After screening various proteases for enzymatic pretreatment, the Box-Behnken design (BBD) was employed to optimize the extraction conditions. The optimized parameters, ultrasound duration of 75&#xa0;min, ultrasound power of 371&#xa0;W, enzyme amount of 1% (w/w of RCO), and incubation time of 81&#xa0;min, yielded 14.67 ± 0.06% oil, a result consistent with the predicted value of 14.7%. HPLC analysis revealed that the RCOO extracted by UAEE contained a higher proportion of unsaturated fatty acids compared to the conventional method. Furthermore, antioxidant assessments (DPPH and ABTS radical scavenging assays) demonstrated that UAEE-derived oil exhibited superior antioxidant activity compared to oils obtained via ultrasound extraction (UE) or supercritical fluid extraction (SFE). These results establish UAEE as an efficient and sustainable approach for producing high-quality RCOO with enhanced bioactive properties.</p> Graphical abstract <p></p>

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Ultrasound-assisted enzymatic extraction of Rana chensinensis ovum oil and optimization using response surface methodology

  • 期刊论文

  • 发表日期：

    2026年2月17日

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  • Mengyuan Li,
  • Hang Li,
  • Manman Zhang,
  • Tong Wang,
  • Yongsheng Wang,
  • Zhihan Wang,
  • Shihan Wang

摘要

This work developed an ultrasound-assisted enzymatic extraction (UAEE) method for producing Rana chensinensis ovum oil (RCOO), a valuable source of omega-3 and omega-6 fatty acids. After screening various proteases for enzymatic pretreatment, the Box-Behnken design (BBD) was employed to optimize the extraction conditions. The optimized parameters, ultrasound duration of 75 min, ultrasound power of 371 W, enzyme amount of 1% (w/w of RCO), and incubation time of 81 min, yielded 14.67 ± 0.06% oil, a result consistent with the predicted value of 14.7%. HPLC analysis revealed that the RCOO extracted by UAEE contained a higher proportion of unsaturated fatty acids compared to the conventional method. Furthermore, antioxidant assessments (DPPH and ABTS radical scavenging assays) demonstrated that UAEE-derived oil exhibited superior antioxidant activity compared to oils obtained via ultrasound extraction (UE) or supercritical fluid extraction (SFE). These results establish UAEE as an efficient and sustainable approach for producing high-quality RCOO with enhanced bioactive properties.

Graphical abstract