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<p>This study aimed to elevate the recovery of bioactive compounds from a cold-pressed defatted rice bran using ethanol-enhanced mild subcritical alkaline water (m-SAWE<sub>tOH</sub>) treatment. Optimal conditions of m-SAWE<sub>tOH</sub> to obtain extracts rich in protein were investigated. Three variables were assessed using a response surface methodology (RSM) in a Box-Behnken Design (BBD) with extraction temperature (x<sub>1</sub>, 110–120&#xa0;°C), time (x<sub>2</sub>, 30–90&#xa0;min), and ethanol level (x<sub>3</sub>, 0–70%). The protein-rich sediment (Prot-Sed) was precipitated by adjusting the extracted supernatant to 75% ethanol. The optimum&#xa0;m-SAWE<sub>tOH</sub> conditions for the Prot-Sed extraction were 120&#xa0;°C, 38.9% ethanol, for 90&#xa0;min. Ethanol enhanced the m-SAW, providing the Prot-Sed with higher protein, total phenolic content, and antioxidant activity (FRAP assay). Additionally, the 2-step process of m-SAWE<sub>tOH</sub> and protease hydrolysis increased the recovery of protein and phenolics by 1.93-fold and 10.42-fold, respectively, concomitant with an increase in antioxidant capacity. Amino acids (His, Leu, Met, Trp, and Val) and polyphenols (protocatechuic acid, <i>p</i>-coumaric acid, ferulic acid, and sinapic acid) possessing antioxidant capacity were presented in the Prot-Sed hydrolysate. The findings demonstrated that m-SAWE<sub>tOH</sub> is a green process that improves the release of bioactive components, adding value to biomaterials.</p>

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Obtaining proteins and protein hydrolysate antioxidants from cold-pressed defatted rice Bran by ethanol-modified mild subcritical water extraction

  • 期刊论文

  • 发表日期：

    2026年2月17日

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  • Sukrichaya Hemathulin,
  • Wilatsana Posri,
  • Araya Chaoruangrit,
  • Supawan Thawornchinsombut

摘要

This study aimed to elevate the recovery of bioactive compounds from a cold-pressed defatted rice bran using ethanol-enhanced mild subcritical alkaline water (m-SAWEtOH) treatment. Optimal conditions of m-SAWEtOH to obtain extracts rich in protein were investigated. Three variables were assessed using a response surface methodology (RSM) in a Box-Behnken Design (BBD) with extraction temperature (x1, 110–120 °C), time (x2, 30–90 min), and ethanol level (x3, 0–70%). The protein-rich sediment (Prot-Sed) was precipitated by adjusting the extracted supernatant to 75% ethanol. The optimum m-SAWEtOH conditions for the Prot-Sed extraction were 120 °C, 38.9% ethanol, for 90 min. Ethanol enhanced the m-SAW, providing the Prot-Sed with higher protein, total phenolic content, and antioxidant activity (FRAP assay). Additionally, the 2-step process of m-SAWEtOH and protease hydrolysis increased the recovery of protein and phenolics by 1.93-fold and 10.42-fold, respectively, concomitant with an increase in antioxidant capacity. Amino acids (His, Leu, Met, Trp, and Val) and polyphenols (protocatechuic acid, p-coumaric acid, ferulic acid, and sinapic acid) possessing antioxidant capacity were presented in the Prot-Sed hydrolysate. The findings demonstrated that m-SAWEtOH is a green process that improves the release of bioactive components, adding value to biomaterials.