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<p>The rise of antibiotic-resistant bacteria has led researchers to explore alternative antimicrobial compounds, such as those found in plants. In this study, pulsed electric fields and subcritical water were used to extract and investigate the radical scavenging ability and phenolic compounds of frankincense gum. The extracted compounds were identified using HPLC and compared to those obtained from traditional maceration methods. Also, antibacterial effects of extracts against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> bacteria were investigated. The results showed that the subcritical water extraction method had the highest efficiency, with the highest extraction efficiency reached at 160&#xa0;°C for 5&#xa0;min. The subcritical water extracts also exhibited greater radical scavenging power compared to other methods. Gallic acid was the most commonly identified compound, with the highest amount (40.64&#xa0;µg/ml) found in the subcritical water extract. Moreover, myricetin was only extractable using the subcritical water method. These findings suggest that subcritical water extraction could be a promising method for obtaining bioactive compounds from frankincense gum. The microbiological results showed that the effects of extracts obtained from maceration, Pulsed Electric Field (PEF) and subcritical water methods on <i>Staphylococcus aureus</i> were greater than on <i>Escherichia coli</i>.</p>

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Comparison of pulsed electric field and subcritical water techniques on the phenolic profile, antimicrobial and antioxidant properties of frankincense gum

  • 期刊论文

  • 发表日期：

    2026年2月18日

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  • Sahar Golchin,
  • Bahareh Hajirostamloo,
  • Zohreh Didar,
  • Morteza Mohammadi,
  • Mohsen Vazifedoost

摘要

The rise of antibiotic-resistant bacteria has led researchers to explore alternative antimicrobial compounds, such as those found in plants. In this study, pulsed electric fields and subcritical water were used to extract and investigate the radical scavenging ability and phenolic compounds of frankincense gum. The extracted compounds were identified using HPLC and compared to those obtained from traditional maceration methods. Also, antibacterial effects of extracts against Escherichia coli and Staphylococcus aureus bacteria were investigated. The results showed that the subcritical water extraction method had the highest efficiency, with the highest extraction efficiency reached at 160 °C for 5 min. The subcritical water extracts also exhibited greater radical scavenging power compared to other methods. Gallic acid was the most commonly identified compound, with the highest amount (40.64 µg/ml) found in the subcritical water extract. Moreover, myricetin was only extractable using the subcritical water method. These findings suggest that subcritical water extraction could be a promising method for obtaining bioactive compounds from frankincense gum. The microbiological results showed that the effects of extracts obtained from maceration, Pulsed Electric Field (PEF) and subcritical water methods on Staphylococcus aureus were greater than on Escherichia coli.