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<p>Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) plays a crucial role in ensuring product quality, safety, and stability in the dairy industry. Recognizing the importance of H<sub>2</sub>O<sub>2</sub> sensing in the dairy sector, this study focuses on the development of a highly sensitive nanofabricated sensor for detecting H<sub>2</sub>O<sub>2</sub> in milk. The sensor is composed of reduced graphene oxide nanosheets (rGO), thionine (Thi), and gold nanoparticles (GNPs) that are electrochemically deposited on an indium tin oxide (ITO)-coated glass substrate, forming the rGO/GNP/Thi/ITO composite. The electrochemical deposition and subsequent H<sub>2</sub>O<sub>2</sub> sensing were conducted using chronoamperometry, cyclic voltammetry, and electrochemical impedance spectroscopy. To achieve selective detection of H<sub>2</sub>O<sub>2</sub>, the peroxidase enzyme was immobilized on the modified electrode surface. Electrochemical characterization demonstrated excellent conductivity and electron transfer properties of the sensor. The developed sensor is highly sensitive with a limit of detection of 66 µM and a wide linear range from 0.05 to 60 mM. The milk samples showed satisfactory recovery rates of H<sub>2</sub>O<sub>2,</sub> with the sensor demonstrating high stability and reproducibility, even after 4 weeks of use. These findings highlight the potential of the rGO/GNP/Thi/ITO nanocomposite as a reliable and cost-effective tool for monitoring H<sub>2</sub>O<sub>2</sub> in milk.</p>

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Reduced graphene organo-metal nanocomposite for the electrochemical detection of hydrogen peroxide in milk

  • 期刊论文

  • 发表日期：

    2025年10月9日

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  • Abhishek Ramachandra Panigrahi,
  • Chandrasekhar Pattanaik,
  • Pooja Yadav,
  • Samir Kumar Beura,
  • Jyoti Singh,
  • Abhishek Kumar Maurya,
  • Nisha Yadav,
  • Sunil Mittal,
  • Sunil Kumar Singh

摘要

Hydrogen peroxide (H2O2) plays a crucial role in ensuring product quality, safety, and stability in the dairy industry. Recognizing the importance of H2O2 sensing in the dairy sector, this study focuses on the development of a highly sensitive nanofabricated sensor for detecting H2O2 in milk. The sensor is composed of reduced graphene oxide nanosheets (rGO), thionine (Thi), and gold nanoparticles (GNPs) that are electrochemically deposited on an indium tin oxide (ITO)-coated glass substrate, forming the rGO/GNP/Thi/ITO composite. The electrochemical deposition and subsequent H2O2 sensing were conducted using chronoamperometry, cyclic voltammetry, and electrochemical impedance spectroscopy. To achieve selective detection of H2O2, the peroxidase enzyme was immobilized on the modified electrode surface. Electrochemical characterization demonstrated excellent conductivity and electron transfer properties of the sensor. The developed sensor is highly sensitive with a limit of detection of 66 µM and a wide linear range from 0.05 to 60 mM. The milk samples showed satisfactory recovery rates of H2O2, with the sensor demonstrating high stability and reproducibility, even after 4 weeks of use. These findings highlight the potential of the rGO/GNP/Thi/ITO nanocomposite as a reliable and cost-effective tool for monitoring H2O2 in milk.