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<p>Foodborne pathogens, especially <i>Staphylococcus aureus</i> (<i>S. aureus</i>), pose a major risk to public health. The currently employed methods to detect such a microorganism are often time-consuming and require specialized equipment. To overcome these obstacles, we designed a nanozyme-based colorimetric aptasensor that employs a simple platform to facilitate the rapid detection of <i>S. aureus</i> in milk. It utilizes a DNA aptamer functionalized NiCoFe-layered double hydroxide (LDH) nanoparticles that can bind specifically to the <i>S. aureus</i> outer membrane. NiCoFe-LDH nanoparticles are utilized as peroxidase enzyme mimics that can oxidize the TMB substrate leading to a blue color. The DNA aptamers’ binding on the surface of NiCoFe-LDH led to an enhancement of the nanozyme activity. Hence, upon the presence of the <i>S. aureus</i>, the aptamers detached from the NiCoFe-LDH NPs to bind to their targeted molecule resulting in lower color intensity. Under optimal conditions, the aptasensor could detect 10 CFU/mL of <i>S. aureus</i> with high specificity. To our understanding, this is the first report of an aptasensor based on NiCoFe-LDH nanozyme for the detection of <i>S. aureus</i>.</p>

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A novel NiCoFe LDH-based aptasensor for rapid colorimetric detection of Staphylococcus aureus in milk

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月10日

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  • Gamal M. Hassan,
  • Arafa M. S. Meshref,
  • Mohamed M. A. Zeinhom,
  • Amany R. Abdel-Wahed,
  • Dalia M. El-Husseini

摘要

Foodborne pathogens, especially Staphylococcus aureus (S. aureus), pose a major risk to public health. The currently employed methods to detect such a microorganism are often time-consuming and require specialized equipment. To overcome these obstacles, we designed a nanozyme-based colorimetric aptasensor that employs a simple platform to facilitate the rapid detection of S. aureus in milk. It utilizes a DNA aptamer functionalized NiCoFe-layered double hydroxide (LDH) nanoparticles that can bind specifically to the S. aureus outer membrane. NiCoFe-LDH nanoparticles are utilized as peroxidase enzyme mimics that can oxidize the TMB substrate leading to a blue color. The DNA aptamers’ binding on the surface of NiCoFe-LDH led to an enhancement of the nanozyme activity. Hence, upon the presence of the S. aureus, the aptamers detached from the NiCoFe-LDH NPs to bind to their targeted molecule resulting in lower color intensity. Under optimal conditions, the aptasensor could detect 10 CFU/mL of S. aureus with high specificity. To our understanding, this is the first report of an aptasensor based on NiCoFe-LDH nanozyme for the detection of S. aureus.