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<p>The present investigation mathematically modeled the inactivation of polyphenol oxidase (PPO) and peroxidase (POD) enzymes in pineapple pulp. The pineapple pulp was exposed to dielectric barrier discharge (DBD) plasma at 15, 20, 25, and 30&#xa0;kV with treatment times of 3, 6, 9, 12, and 15&#xa0;min. The result showed that, after 15&#xa0;min of plasma exposure, dielectric barrier discharge (DBD) plasma exposure of 30&#xa0;kV was sufficient to reach the maximum inactivation of PPO and POD in pineapple pulp up to 90% and 87%, respectively. The regression coefficient of the Peleg and Two-fraction kinetic model was suitable for the model fitting. However, the RMSE/error in both models indicates the limiting characteristics of the fitted models over experimental observations. Based on the model validation, the Peleg model was more adequate for describing both the PPO and POD enzymes in pineapple pulp with R<sup>2</sup> &gt; 0.99, RMSE/error &lt; 0.041, A<sub>f</sub> and B<sub>f</sub> ~ 1), AIC ≥ − 113.348, and BIC ≥ − 4.560.</p>

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Modelling the enzymatic activity of pineapple pulp affected by DBD voltage and treatment time of atmospheric cold plasma

  • 期刊论文

  • 发表日期：

    2025年9月17日

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  • Arjuara Begum,
  • Sahijul Islam,
  • Amardeep Kumar,
  • Brijesh Srivastava

摘要

The present investigation mathematically modeled the inactivation of polyphenol oxidase (PPO) and peroxidase (POD) enzymes in pineapple pulp. The pineapple pulp was exposed to dielectric barrier discharge (DBD) plasma at 15, 20, 25, and 30 kV with treatment times of 3, 6, 9, 12, and 15 min. The result showed that, after 15 min of plasma exposure, dielectric barrier discharge (DBD) plasma exposure of 30 kV was sufficient to reach the maximum inactivation of PPO and POD in pineapple pulp up to 90% and 87%, respectively. The regression coefficient of the Peleg and Two-fraction kinetic model was suitable for the model fitting. However, the RMSE/error in both models indicates the limiting characteristics of the fitted models over experimental observations. Based on the model validation, the Peleg model was more adequate for describing both the PPO and POD enzymes in pineapple pulp with R2 > 0.99, RMSE/error < 0.041, Af and Bf ~ 1), AIC ≥ − 113.348, and BIC ≥ − 4.560.